Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences

Laura S. Burrack, Shelly E. Applen Clancey, Jeremy M Chacon, Melissa K Gardner, Judith Berman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The establishment and maintenance of higher-order structure at centromeres is essential for accurate chromosome segregation. The monopolin complex is thought to cross-link multiple kinetochore complexes to prevent merotelic attachments that result in chromosome missegregation. This model is based on structural analysis and the requirement that monopolin execute mitotic and meiotic chromosome segregation in Schizosaccharomyces pombe, which has more than one kinetochore-microtubule attachment/centromere, and co-orient sister chromatids in meiosis I in Saccharomyces cerevisiae. Recent data from S. pombe suggest an alternative possibility: that the recruitment of condensin is the primary function of monopolin. Here we test these models using the yeast Candida albicans. C. albicans cells lacking monopolin exhibit defects in chromosome segregation, increased distance between centromeres, and decreased stability of several types of repeat DNA. Of note, changing kinetochore-microtubule copy number from one to more than one kinetochore-microtubule/centromere does not alter the requirement for monopolin. Furthermore, monopolin recruits condensin to C. albicans centromeres, and overexpression of condensin suppresses chromosome segregation defects in strains lacking monopolin. We propose that the key function of monopolin is to recruit condensin in order to promote the assembly of higher-order structure at centromere and repetitive DNA.

Original languageEnglish (US)
Pages (from-to)2807-2819
Number of pages13
JournalMolecular Biology of the Cell
Volume24
Issue number18
DOIs
StatePublished - Sep 15 2013

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Centromere
Nucleic Acid Repetitive Sequences
Kinetochores
Chromosome Segregation
DNA
Candida albicans
Microtubules
Schizosaccharomyces
Chromatids
Meiosis
Saccharomyces cerevisiae
condensin complexes
Chromosomes
Yeasts

Cite this

Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences. / Burrack, Laura S.; Applen Clancey, Shelly E.; Chacon, Jeremy M; Gardner, Melissa K; Berman, Judith.

In: Molecular Biology of the Cell, Vol. 24, No. 18, 15.09.2013, p. 2807-2819.

Research output: Contribution to journalArticle

Burrack, Laura S. ; Applen Clancey, Shelly E. ; Chacon, Jeremy M ; Gardner, Melissa K ; Berman, Judith. / Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences. In: Molecular Biology of the Cell. 2013 ; Vol. 24, No. 18. pp. 2807-2819.
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