Monolayer culture of human endometrium: Methods of culture and identification of cell types

V. A. Varma, Susan A. Melin, Thomas A. Adamec, B. Hugh Dorman, Jill M. Siegfried, Leslie A. Walton, Charles N. Carney, Carol R. Norton, David G. Kaufman

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Monolayer cultures can be established from human endometrial tissue after enzymatic dispersal into isolated glands or single cells. Three cell types that have distinct morphology by light and electron microscopy are observed in the resulting primary cultures. One cell type, an elongated spindle cell, is similar in appearance to fibroblasts derived from other tissues. A second cell type forms colonies of tightly cohesive cells, ranging in shape from oval to polygonal. These cells have typical organelles and junctional complexes characteristic of epithelial cells from the endometrium. The third cell type assumes a pavement-like appearance composed of polygonal cells when viewed by phase contrast microscopy, but lacks distinctive ultrastructural features of epithelial cells. These cells in culture resemble the endometrial stromal cell, the predominant cell type of the human endometrium in vivo. The epithelial cell does not survive subculturing but the other two cell types can be passaged through several generations and can be stored in liquid nitrogen and subsequently returned to culture.

Original languageEnglish (US)
Pages (from-to)911-918
Number of pages8
JournalIn Vitro
Volume18
Issue number11
DOIs
StatePublished - Nov 1 1982

Fingerprint

Endometrium
Monolayers
Cell Culture Techniques
Tissue
Liquid nitrogen
Fibroblasts
Pavements
Electron microscopy
Optical microscopy
Microscopic examination
Epithelial Cells
Phase-Contrast Microscopy
Stromal Cells
Organelles
Electron Microscopy
Nitrogen
Light

Keywords

  • human endometrium
  • stromal cell

Cite this

Varma, V. A., Melin, S. A., Adamec, T. A., Dorman, B. H., Siegfried, J. M., Walton, L. A., ... Kaufman, D. G. (1982). Monolayer culture of human endometrium: Methods of culture and identification of cell types. In Vitro, 18(11), 911-918. https://doi.org/10.1007/BF02796347

Monolayer culture of human endometrium : Methods of culture and identification of cell types. / Varma, V. A.; Melin, Susan A.; Adamec, Thomas A.; Dorman, B. Hugh; Siegfried, Jill M.; Walton, Leslie A.; Carney, Charles N.; Norton, Carol R.; Kaufman, David G.

In: In Vitro, Vol. 18, No. 11, 01.11.1982, p. 911-918.

Research output: Contribution to journalArticle

Varma, VA, Melin, SA, Adamec, TA, Dorman, BH, Siegfried, JM, Walton, LA, Carney, CN, Norton, CR & Kaufman, DG 1982, 'Monolayer culture of human endometrium: Methods of culture and identification of cell types', In Vitro, vol. 18, no. 11, pp. 911-918. https://doi.org/10.1007/BF02796347
Varma, V. A. ; Melin, Susan A. ; Adamec, Thomas A. ; Dorman, B. Hugh ; Siegfried, Jill M. ; Walton, Leslie A. ; Carney, Charles N. ; Norton, Carol R. ; Kaufman, David G. / Monolayer culture of human endometrium : Methods of culture and identification of cell types. In: In Vitro. 1982 ; Vol. 18, No. 11. pp. 911-918.
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