Monocyte-induced potentiation of bovine fetal thymocyte mitogenic responses to concanavalin A

S. P. Kumar; D. W. Johnson; F. C. Okino; C. C. Muscoplat

doi:10.1016/0165-2427(80)90004-5

Monocyte-induced potentiation of bovine fetal thymocyte mitogenic responses to concanavalin A

S. P. Kumar, D. W. Johnson, F. C. Okino, C. C. Muscoplat

Administration (CFANS)

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Peripheral blood monocytes significantly potentiated the mitogenic response of bovine fetal thymocytes to Concanavalin A as measured by incorporation of [³H] thymidine into cellular DNA. Mononuclear cells obtained from either normal or Mycobacterium bovis sensitized cattle were cultured with or without purified protein derivative (PPD) for 24 hours at which time bovine fetal thymocytes and concanavalin A were added. After 3 days of culture, both activated or non-activated monocytes significantly potentiated Con A-induced blastogenic responses. Depletion of monocytes from thymocyte cultures completely abrogated thymocyte responses to Concanavalin A.

Original language	English (US)
Pages (from-to)	145-152
Number of pages	8
Journal	Veterinary immunology and immunopathology
Volume	1
Issue number	2
DOIs	https://doi.org/10.1016/0165-2427(80)90004-5
State	Published - Feb 1980

Bibliographical note

Funding Information:
Supported in part by the Minnesota Agricultural Experiment Station, the Cooperative Research Service and Veterinary Services, U.S. Department of Agriculture, Hyattsville, MD, and the Ministry of Education and Social Welfare, Government of India, New Delhi, India.

Publisher link

10.1016/0165-2427(80)90004-5

Find It

Find It at U of M Libraries

Cite this

@article{ba1bae8f44644cfa99d69ae2404f78d9,

title = "Monocyte-induced potentiation of bovine fetal thymocyte mitogenic responses to concanavalin A",

abstract = "Peripheral blood monocytes significantly potentiated the mitogenic response of bovine fetal thymocytes to Concanavalin A as measured by incorporation of [3H] thymidine into cellular DNA. Mononuclear cells obtained from either normal or Mycobacterium bovis sensitized cattle were cultured with or without purified protein derivative (PPD) for 24 hours at which time bovine fetal thymocytes and concanavalin A were added. After 3 days of culture, both activated or non-activated monocytes significantly potentiated Con A-induced blastogenic responses. Depletion of monocytes from thymocyte cultures completely abrogated thymocyte responses to Concanavalin A.",

author = "Kumar, {S. P.} and Johnson, {D. W.} and Okino, {F. C.} and Muscoplat, {C. C.}",

note = "Funding Information: Supported in part by the Minnesota Agricultural Experiment Station, the Cooperative Research Service and Veterinary Services, U.S. Department of Agriculture, Hyattsville, MD, and the Ministry of Education and Social Welfare, Government of India, New Delhi, India.",

year = "1980",

month = feb,

doi = "10.1016/0165-2427(80)90004-5",

language = "English (US)",

volume = "1",

pages = "145--152",

journal = "Veterinary immunology and immunopathology",

issn = "0165-2427",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Monocyte-induced potentiation of bovine fetal thymocyte mitogenic responses to concanavalin A

AU - Kumar, S. P.

AU - Johnson, D. W.

AU - Okino, F. C.

AU - Muscoplat, C. C.

N1 - Funding Information: Supported in part by the Minnesota Agricultural Experiment Station, the Cooperative Research Service and Veterinary Services, U.S. Department of Agriculture, Hyattsville, MD, and the Ministry of Education and Social Welfare, Government of India, New Delhi, India.

PY - 1980/2

Y1 - 1980/2

N2 - Peripheral blood monocytes significantly potentiated the mitogenic response of bovine fetal thymocytes to Concanavalin A as measured by incorporation of [3H] thymidine into cellular DNA. Mononuclear cells obtained from either normal or Mycobacterium bovis sensitized cattle were cultured with or without purified protein derivative (PPD) for 24 hours at which time bovine fetal thymocytes and concanavalin A were added. After 3 days of culture, both activated or non-activated monocytes significantly potentiated Con A-induced blastogenic responses. Depletion of monocytes from thymocyte cultures completely abrogated thymocyte responses to Concanavalin A.

AB - Peripheral blood monocytes significantly potentiated the mitogenic response of bovine fetal thymocytes to Concanavalin A as measured by incorporation of [3H] thymidine into cellular DNA. Mononuclear cells obtained from either normal or Mycobacterium bovis sensitized cattle were cultured with or without purified protein derivative (PPD) for 24 hours at which time bovine fetal thymocytes and concanavalin A were added. After 3 days of culture, both activated or non-activated monocytes significantly potentiated Con A-induced blastogenic responses. Depletion of monocytes from thymocyte cultures completely abrogated thymocyte responses to Concanavalin A.

UR - http://www.scopus.com/inward/record.url?scp=16644377962&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16644377962&partnerID=8YFLogxK

U2 - 10.1016/0165-2427(80)90004-5

DO - 10.1016/0165-2427(80)90004-5

M3 - Article

C2 - 15612260

AN - SCOPUS:16644377962

SN - 0165-2427

VL - 1

SP - 145

EP - 152

JO - Veterinary immunology and immunopathology

JF - Veterinary immunology and immunopathology

IS - 2

ER -

Monocyte-induced potentiation of bovine fetal thymocyte mitogenic responses to concanavalin A

Abstract

Bibliographical note

Publisher link

Find It

Other files and links

Fingerprint

Cite this