Monoclonal antibody profiling of cell surface proteins associated with the viral biofilms on HTLV-1 transformed cells

A. Tarasevich; A. Filatov; A. Pichugin; D. Mazurov

doi:10.4149/av_2015_03_247

Monoclonal antibody profiling of cell surface proteins associated with the viral biofilms on HTLV-1 transformed cells

A. Tarasevich
, A. Filatov
, A. Pichugin
, D. Mazurov

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Human T lymphotropic virus 1 (HTLV-1) is a pathogenic retrovirus that spreads predominantly via cell-to-cell contact. Two models of cell-to-cell virus transmission are proposed: virological synapse (VS) and viral biofilms (VB). Both infectious structures can be involved in transmission and synergistically enhance HTLV-1 spread between cells. Although transmission of virus via VB has been reported, the molecular composition of VB remains poorly understood. In this study we generated new anti-VB monoclonal antibodies (MAbs) and screened them along with a panel of anti-human cluster of differentiation (CD) MAbs to select antigens associated with VB. Among four MAbs generated against VB, two MAbs were identified as anti-CD25 (IL-2RA). We found that antigens CD4, CD150, CD25, CD70, and CD80 were enriched in VB. We also determined that expression of viral protein Tax, a central molecule in HTLV-1 transmission, upregulates intercellular adhesion molecule 1 (ICAM-1), CD95, CD25, CD70, and CD80. Whether these antigens are essential for VB formation and HTLV-1 infection remains unknown and will be determined in further experiments.

Original language	English (US)
Pages (from-to)	247-256
Number of pages	10
Journal	Acta Virologica
Volume	59
Issue number	3
DOIs	https://doi.org/10.4149/av_2015_03_247
State	Published - 2015
Externally published	Yes

Bibliographical note

Funding Information:
This work has been supported by the grant of Russian Foundation for Basic Research (RFBR No. 12-04-00415-a), partially by the NIH-RFBR grant No. 13-04-91449, and by the grant of Russian Science Foundation (RSF No. 15-15-00135). We thank Alexander Sapojnikov and Elena Kovalenko from the Institute of Bioorganic Chemistry (Moscow, Russia) for their help with confocal imaging, and Olga Nikolaitchik from NCI-Frederick (Frederick, MD) for critical reading of the manuscript.

Funding Information:
Acknowledgements. This work has been supported by the grant of Russian Foundation for Basic Research (RFBR № 12-04-00415-a), partially by the NIH-RFBR grant № 13-04-91449, and by the grant of Russian Science Foundation (RSF № 15-15-00135). We thank Alexander Sapojnikov and Elena Kovalenko from the Institute of Bioorganic Chemistry (Moscow, Russia) for their help with confocal imaging, and Olga Nikolaitchik from NCI-Frederick (Frederick, MD) for critical reading of the manuscript.

Keywords

Biofilms
CD antigen
HTLV-1
Monoclonal antibody

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title = "Monoclonal antibody profiling of cell surface proteins associated with the viral biofilms on HTLV-1 transformed cells",

abstract = "Human T lymphotropic virus 1 (HTLV-1) is a pathogenic retrovirus that spreads predominantly via cell-to-cell contact. Two models of cell-to-cell virus transmission are proposed: virological synapse (VS) and viral biofilms (VB). Both infectious structures can be involved in transmission and synergistically enhance HTLV-1 spread between cells. Although transmission of virus via VB has been reported, the molecular composition of VB remains poorly understood. In this study we generated new anti-VB monoclonal antibodies (MAbs) and screened them along with a panel of anti-human cluster of differentiation (CD) MAbs to select antigens associated with VB. Among four MAbs generated against VB, two MAbs were identified as anti-CD25 (IL-2RA). We found that antigens CD4, CD150, CD25, CD70, and CD80 were enriched in VB. We also determined that expression of viral protein Tax, a central molecule in HTLV-1 transmission, upregulates intercellular adhesion molecule 1 (ICAM-1), CD95, CD25, CD70, and CD80. Whether these antigens are essential for VB formation and HTLV-1 infection remains unknown and will be determined in further experiments.",

keywords = "Biofilms, CD antigen, HTLV-1, Monoclonal antibody",

author = "A. Tarasevich and A. Filatov and A. Pichugin and D. Mazurov",

note = "Funding Information: This work has been supported by the grant of Russian Foundation for Basic Research (RFBR No. 12-04-00415-a), partially by the NIH-RFBR grant No. 13-04-91449, and by the grant of Russian Science Foundation (RSF No. 15-15-00135). We thank Alexander Sapojnikov and Elena Kovalenko from the Institute of Bioorganic Chemistry (Moscow, Russia) for their help with confocal imaging, and Olga Nikolaitchik from NCI-Frederick (Frederick, MD) for critical reading of the manuscript. Funding Information: Acknowledgements. This work has been supported by the grant of Russian Foundation for Basic Research (RFBR № 12-04-00415-a), partially by the NIH-RFBR grant № 13-04-91449, and by the grant of Russian Science Foundation (RSF № 15-15-00135). We thank Alexander Sapojnikov and Elena Kovalenko from the Institute of Bioorganic Chemistry (Moscow, Russia) for their help with confocal imaging, and Olga Nikolaitchik from NCI-Frederick (Frederick, MD) for critical reading of the manuscript.",

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AU - Pichugin, A.

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N1 - Funding Information: This work has been supported by the grant of Russian Foundation for Basic Research (RFBR No. 12-04-00415-a), partially by the NIH-RFBR grant No. 13-04-91449, and by the grant of Russian Science Foundation (RSF No. 15-15-00135). We thank Alexander Sapojnikov and Elena Kovalenko from the Institute of Bioorganic Chemistry (Moscow, Russia) for their help with confocal imaging, and Olga Nikolaitchik from NCI-Frederick (Frederick, MD) for critical reading of the manuscript. Funding Information: Acknowledgements. This work has been supported by the grant of Russian Foundation for Basic Research (RFBR № 12-04-00415-a), partially by the NIH-RFBR grant № 13-04-91449, and by the grant of Russian Science Foundation (RSF № 15-15-00135). We thank Alexander Sapojnikov and Elena Kovalenko from the Institute of Bioorganic Chemistry (Moscow, Russia) for their help with confocal imaging, and Olga Nikolaitchik from NCI-Frederick (Frederick, MD) for critical reading of the manuscript.

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N2 - Human T lymphotropic virus 1 (HTLV-1) is a pathogenic retrovirus that spreads predominantly via cell-to-cell contact. Two models of cell-to-cell virus transmission are proposed: virological synapse (VS) and viral biofilms (VB). Both infectious structures can be involved in transmission and synergistically enhance HTLV-1 spread between cells. Although transmission of virus via VB has been reported, the molecular composition of VB remains poorly understood. In this study we generated new anti-VB monoclonal antibodies (MAbs) and screened them along with a panel of anti-human cluster of differentiation (CD) MAbs to select antigens associated with VB. Among four MAbs generated against VB, two MAbs were identified as anti-CD25 (IL-2RA). We found that antigens CD4, CD150, CD25, CD70, and CD80 were enriched in VB. We also determined that expression of viral protein Tax, a central molecule in HTLV-1 transmission, upregulates intercellular adhesion molecule 1 (ICAM-1), CD95, CD25, CD70, and CD80. Whether these antigens are essential for VB formation and HTLV-1 infection remains unknown and will be determined in further experiments.

AB - Human T lymphotropic virus 1 (HTLV-1) is a pathogenic retrovirus that spreads predominantly via cell-to-cell contact. Two models of cell-to-cell virus transmission are proposed: virological synapse (VS) and viral biofilms (VB). Both infectious structures can be involved in transmission and synergistically enhance HTLV-1 spread between cells. Although transmission of virus via VB has been reported, the molecular composition of VB remains poorly understood. In this study we generated new anti-VB monoclonal antibodies (MAbs) and screened them along with a panel of anti-human cluster of differentiation (CD) MAbs to select antigens associated with VB. Among four MAbs generated against VB, two MAbs were identified as anti-CD25 (IL-2RA). We found that antigens CD4, CD150, CD25, CD70, and CD80 were enriched in VB. We also determined that expression of viral protein Tax, a central molecule in HTLV-1 transmission, upregulates intercellular adhesion molecule 1 (ICAM-1), CD95, CD25, CD70, and CD80. Whether these antigens are essential for VB formation and HTLV-1 infection remains unknown and will be determined in further experiments.

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AN - SCOPUS:84952319397

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Monoclonal antibody profiling of cell surface proteins associated with the viral biofilms on HTLV-1 transformed cells

Abstract

Bibliographical note

Keywords

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