TY - JOUR
T1 - Monoaminergic changes in locus coeruleus and dorsal raphe nucleus following noradrenaline depletion
AU - Cassano, Tommaso
AU - Gaetani, Silvana
AU - Morgese, Maria Grazia
AU - MacHeda, Teresa
AU - Laconca, Leonardo
AU - Dipasquale, Pasqua
AU - Taltavull, Juan
AU - Shippenberg, Toni S.
AU - Cuomo, Vincenzo
AU - Gobbi, Gabriella
PY - 2009/8
Y1 - 2009/8
N2 - The goal of our study was to assess the monoaminergic changes in locus coeruleus (LC) and dorsal raphe nucleus (DRN) following noradrenaline (NA) depletion. Seven days after a single N-(2-chloroethyl)-N-ethyl-2- bromobenzylamine (DSP-4) intraperitoneal administration in mice, we observed a decrease of NA in both the LC and DRN, as well as in prefrontal cortex (PFC) and hippocampus (HIPP). Moreover, an increase of serotonin (5-HT) and 5-hydroxyindolacetic acid (5-HIAA) was detected at LC level, while no change was found in DRN. DSP-4 also caused a significant decrease of dopamine (DA) tissue content in HIPP and DRN, without affecting the LC and the PFC. A decrease of DA metabolite, homovanillic acid (HVA), was found in the DRN of NA-depleted mice. These results highlight that the neurotoxic action of DSP-4 is not restricted to LC terminal projections but also involves NA depletion at the cell body level, where it is paralleled by adaptive changes in both serotonergic and dopaminergic systems.
AB - The goal of our study was to assess the monoaminergic changes in locus coeruleus (LC) and dorsal raphe nucleus (DRN) following noradrenaline (NA) depletion. Seven days after a single N-(2-chloroethyl)-N-ethyl-2- bromobenzylamine (DSP-4) intraperitoneal administration in mice, we observed a decrease of NA in both the LC and DRN, as well as in prefrontal cortex (PFC) and hippocampus (HIPP). Moreover, an increase of serotonin (5-HT) and 5-hydroxyindolacetic acid (5-HIAA) was detected at LC level, while no change was found in DRN. DSP-4 also caused a significant decrease of dopamine (DA) tissue content in HIPP and DRN, without affecting the LC and the PFC. A decrease of DA metabolite, homovanillic acid (HVA), was found in the DRN of NA-depleted mice. These results highlight that the neurotoxic action of DSP-4 is not restricted to LC terminal projections but also involves NA depletion at the cell body level, where it is paralleled by adaptive changes in both serotonergic and dopaminergic systems.
KW - DSP-4
KW - Dopamine
KW - Dorsal raphe
KW - Locus coeruleus
KW - Noradrenaline
KW - Serotonin
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U2 - 10.1007/s11064-009-9928-5
DO - 10.1007/s11064-009-9928-5
M3 - Article
C2 - 19229609
AN - SCOPUS:67349225035
SN - 0364-3190
VL - 34
SP - 1417
EP - 1426
JO - Neurochemical Research
JF - Neurochemical Research
IS - 8
ER -