Monitoring transport of a recombinant phosphotriesterase in Streptomyces lividans using a pulse-chase system

M. Sathyamoorthy, M. K. Speedie

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A heterologous phosphotriesterase (parathion hydrolase) was previosly shown to be secreted by Streptomyces lividans. To investigate the mechanism of secretion, a system to label the protein and follow its expression and secretion was developed. The recombinant S. lividans was grown first in a defined medium containing [35S]methionine that permitted expression but not secretion. It was then transferred to tryptone/glucose medium with unlabeled methionine for the chase period, during which secretion was observed. The results indicate a relatively slow rate of secretion that is also dependent on the growth medium.

Original languageEnglish (US)
Pages (from-to)493-497
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume43
Issue number3
DOIs
StatePublished - Jul 1995

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