The intracellular metabolism of 3′-azido-3′-deoxythymidine (AZT)-(L)-tryptophan methyl ester phosphoramidate (L-ATO) and AZT-(L)-phenylalanine methyl ester phosphoramidate (L-APO) by the human T-lymphoblastoid cell line CCRF-CEM (CEM-1.3) and peripheral blood mononuclear cell line (PBMC) was investigated with high field 31P NMR spectroscopy. The AZT amino acid phosphoramidates were shown to accumulate intracellularly and to be readily converted into AZT-MP by both tissues types. Thus, the efficient delivery of nucleoside monophosphates to cells can be facilitated by nucleoside phosphoramidate pronucleotides.
Bibliographical noteFunding Information:
The authors are thankful to Dr. Balfour’s group in ACTU unit in the Department of Lab-medicine and Pathology at the University of Minnesota for providing PBMCs. We are also grateful to Dr. David Live and Dr. Beverly Ostrowski for their assistance in NMR technique. NMR instrumentation was provided with funds from the NSF (BIR-961477), the University of Minnesota Medical Scholl, and the Minnesota Medical Foundation. This research is supported by NIH-NCI (CA 89615).
Copyright 2008 Elsevier B.V., All rights reserved.
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