Monitoring starch saccharification using low field NMR

R. Fu, J. Qi, R. Ruan, P. L. Chen, Xiangyang Lin, Xiaofei Ye, Chris Doona, Tom Yang

Research output: Contribution to conferencePaperpeer-review

Abstract

The present study is an attempt to develop a low field NMR based technique for fast analysis of enzymatic saccharification of corn starch. Experiments were first conducted on model samples containing pure starch and glucose to prove the hypothesis. Starch saccharification was carried out using two commercial enzymes, and monitored using CPMG NMR pulse sequence using a low field NMR system (0.5 T, Rl Maran DRX NMR Imaging System). NMR-determined and conventional method-determined glucose concentrations were compared. Data obtained using the CPMG pulse sequence were fitted to a multi-component model. Good linear correlations between NMR relaxation parameters (relaxation time and amplitude) and concentrations of starch and glucose were observed.

Original languageEnglish (US)
StatePublished - 2005
Event2005 ASAE Annual International Meeting - Tampa, FL, United States
Duration: Jul 17 2005Jul 20 2005

Other

Other2005 ASAE Annual International Meeting
Country/TerritoryUnited States
CityTampa, FL
Period7/17/057/20/05

Keywords

  • Ethanol
  • Fermentation
  • Glucose
  • NMR
  • Nuclear magnetic resonance
  • Relaxation
  • Saccharification

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