Monitoring of large B-cell lymphoma and T-zone lymphoma in a dog via flow cytometry

Claire S. Rosenbaum, Davis M. Seelig, Erin N. Burton, Angela D. Gwynn, Jennifer Granick, Hannah R. Able

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A 12-y-old, castrated male Pomeranian dog was presented because of mandibular lymph node (LN) enlargement. Physical examination and a complete blood count revealed generalized lymphadenopathy and moderate lymphocytosis. Fine-needle aspirate cytology revealed expansion of medium lymphocytes in the right mandibular LN and expansion of large lymphocytes in the left popliteal LN. Flow cytometry identified 2 aberrant lymphocyte populations in both LNs, namely a CD5+CD45− T-cell population, and a large CD21+ B-cell population. Flow cytometry of the peripheral blood revealed an identical population of aberrant CD45− T cells. The patient was diagnosed with concurrent T-zone lymphoma and leukemia, and B-cell lymphoma. Multi-agent chemotherapy was instituted, and serial clinical and flow cytometric analysis revealed complete remission of the neoplastic B cells, but persistence of the neoplastic T cells and persistent lymphadenopathy. This case affirms the diagnostic value of flow cytometry and reveals a unique limitation of the RECIST criteria.

Original languageEnglish (US)
Pages (from-to)1008-1012
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Issue number5
StatePublished - Sep 2021

Bibliographical note

Publisher Copyright:
© 2021 The Author(s).


  • B-cell lymphoma
  • RECIST criteria
  • T-zone lymphoma/leukemia
  • cytology
  • dogs
  • flow cytometry
  • lymph node


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