Monitoring collagen transcription by vascular smooth muscle cells in fibrin-based tissue constructs

Justin S. Weinbaum, Jie Qi, Robert T Tranquillo

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Current methods for measuring collagen content in engineered tissues are incompatible with monitoring of collagen production because they require destruction of the tissue. We have implemented a luciferase-based strategy to monitor collagen production noninvasively. Fibrin-based tissue constructs made using vascular smooth muscle cells stably transfected with a collagen I promoter/luciferase transgene developed with collagen content comparable to control cells, but could be imaged noninvasively to follow collagen transcription during tissue growth in vitro. We showed that these cells reported collagen I production at the transcriptional level in response to the growth factor transforming growth factor-β1 and fibrinolytic inhibition by-aminocaproic acid and that these changes were consistent with changes at the mRNA and protein levels. As these cells report collagen changes instantly and without tissue destruction, they will facilitate construct optimization using multiple stimuli to produce functional engineered tissues.

Original languageEnglish (US)
Pages (from-to)459-467
Number of pages9
JournalTissue Engineering - Part C: Methods
Volume16
Issue number3
DOIs
StatePublished - Jun 1 2010

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Transcription
Fibrin
Vascular Smooth Muscle
Collagen
Smooth Muscle Myocytes
Muscle
Cells
Tissue
Monitoring
Luciferases
Aminocaproic Acid
Transforming Growth Factors
Transgenes
Intercellular Signaling Peptides and Proteins
Proteins
Messenger RNA
Acids
Growth

Cite this

Monitoring collagen transcription by vascular smooth muscle cells in fibrin-based tissue constructs. / Weinbaum, Justin S.; Qi, Jie; Tranquillo, Robert T.

In: Tissue Engineering - Part C: Methods, Vol. 16, No. 3, 01.06.2010, p. 459-467.

Research output: Contribution to journalArticle

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