Molecular profiling reveals prognostically significant subtypes of canine lymphoma

A. M. Frantz, A. L. Sarver, D. Ito, T. L. Phang, A. Karimpour-Fard, M. C. Scott, V. E O Valli, K. Lindblad-Toh, K. E. Burgess, B. D. Husbands, M. S. Henson, A. Borgatti, W. C. Kisseberth, L. E. Hunter, M. Breen, T. D. O'Brien, Jaime F. Modiano

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Abstract

We performed genomewide gene expression analysis of 35 samples representing 6 common histologic subtypes of canine lymphoma and bioinformatics analyses to define their molecular characteristics. Three major groups were defined on the basis of gene expression profiles: (1) low-grade T-cell lymphoma, composed entirely by T-zone lymphoma; (2) high-grade T-cell lymphoma, consisting of lymphoblastic T-cell lymphoma and peripheral T-cell lymphoma not otherwise specified; and (3) B-cell lymphoma, consisting of marginal B-cell lymphoma, diffuse large B-cell lymphoma, and Burkitt lymphoma. Interspecies comparative analyses of gene expression profiles also showed that marginal B-cell lymphoma and diffuse large B-cell lymphoma in dogs and humans might represent a continuum of disease with similar drivers. The classification of these diverse tumors into 3 subgroups was prognostically significant, as the groups were directly correlated with event-free survival. Finally, we developed a benchtop diagnostic test based on expression of 4 genes that can robustly classify canine lymphomas into one of these 3 subgroups, enabling a direct clinical application for our results.

Original languageEnglish (US)
Pages (from-to)693-703
Number of pages11
JournalVeterinary pathology
Volume50
Issue number4
DOIs
StatePublished - Aug 1 2013

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Keywords

  • Canine
  • Gene expression profiling
  • Lymphoma subclassification
  • Prognostication

Cite this

Frantz, A. M., Sarver, A. L., Ito, D., Phang, T. L., Karimpour-Fard, A., Scott, M. C., Valli, V. E. O., Lindblad-Toh, K., Burgess, K. E., Husbands, B. D., Henson, M. S., Borgatti, A., Kisseberth, W. C., Hunter, L. E., Breen, M., O'Brien, T. D., & Modiano, J. F. (2013). Molecular profiling reveals prognostically significant subtypes of canine lymphoma. Veterinary pathology, 50(4), 693-703. https://doi.org/10.1177/0300985812465325