Molecular map of the Chlamydomonas reinhardtii nuclear genome

Pushpa Kathir, Matthew LaVoie, William J. Brazelton, Nancy A. Haas, Paul A. Lefebvre, Carolyn D. Silflow

Research output: Contribution to journalArticlepeer-review

94 Scopus citations

Abstract

We have prepared a molecular map of the Chlamydomonas reinhardtii genome anchored to the genetic map. The map consists of 264 markers, including sequence-tagged sites (STS), scored by use of PCR and agarose gel electrophoresis, and restriction fragment length polymorphism markers, scored by use of Southern blot hybridization. All molecular markers tested map to one of the 17 known linkage groups of C. reinhardtii. The map covers approximately 1,000 centimorgans (cM). Any position on the C. reinhardtii genetic map is, on average, within 2 cM of a mapped molecular marker. This molecular map, in combination with the ongoing mapping of bacterial artificial chromosome (BAC) clones and the forthcoming sequence of the C. reinhardtii nuclear genome, should greatly facilitate isolation of genes of interest by using positional cloning methods. In addition, the presence of easily assayed STS markers on each arm of each linkage group should be very useful in mapping new mutations in preparation for positional cloning.

Original languageEnglish (US)
Pages (from-to)362-379
Number of pages18
JournalEukaryotic Cell
Volume2
Issue number2
DOIs
StatePublished - Apr 2003

Fingerprint Dive into the research topics of 'Molecular map of the Chlamydomonas reinhardtii nuclear genome'. Together they form a unique fingerprint.

Cite this