Molecular Insights into the Mechanisms of SUN1 Oligomerization in the Nuclear Envelope

Zeinab Jahed, Darya Fadavi, Uyen T. Vu, Ehsaneddin Asgari, G. W.Gant Luxton, Mohammad R.K. Mofrad

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The LINC complex is found in a wide variety of organisms and is formed by the transluminal interaction between outer- and inner-nuclear-membrane KASH and SUN proteins, respectively. Most extensively studied are SUN1 and SUN2 proteins, which are widely expressed in mammals. Although SUN1 and SUN2 play functionally redundant roles in several cellular processes, more recent studies have revealed diverse and distinct functions for SUN1. While several recent in vitro structural studies have revealed the molecular details of various fragments of SUN2, no such structural information is available for SUN1. Herein, we conduct a systematic analysis of the molecular relationships between SUN1 and SUN2, highlighting key similarities and differences that could lead to clues into their distinct functions. We use a wide range of computational tools, including multiple sequence alignments, homology modeling, molecular docking, and molecular dynamic simulations, to predict structural differences between SUN1 and SUN2, with the goal of understanding the molecular mechanisms underlying SUN1 oligomerization in the nuclear envelope. Our simulations suggest that the structural model of SUN1 is stable in a trimeric state and that SUN1 trimers can associate through their SUN domains to form lateral complexes. We also ask whether SUN1 could adopt an inactive monomeric conformation as seen in SUN2. Our results imply that the KASH binding domain of SUN1 is also inhibited in monomeric SUN1 but through weaker interactions than in monomeric SUN2.

Original languageEnglish (US)
Pages (from-to)1190-1203
Number of pages14
JournalBiophysical journal
Volume114
Issue number5
DOIs
StatePublished - Mar 13 2018

Bibliographical note

Funding Information:
This work was supported by the National Science Foundation through grant CBET-0955291 . In addition, this research used resources of the National Energy Research Scientific Computing Center , a Department of Energy Office of Science user facility supported by the Office of Science of the U.S. Department of Energy under contract No. DE-AC02-05CH11231 .

Publisher Copyright:
© 2018 Biophysical Society

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