Molecular Identification and Antimicrobial Activity of Foliar Endophytic Fungi on the Brazilian Pepper Tree (Schinus terebinthifolius) Reveal New Species of Diaporthe

Germana D. dos Santos, Renata R. Gomes, Rosana Gonçalves, Gheniffer Fornari, Beatriz H.L.N.S. Maia, Claudia Schmidt-Dannert, Francois Gaascht, Chirlei Glienke, Gabriela X. Schneider, Israella R. Colombo, Juliana Degenhardt-Goldbach, João L.M. Pietsch, Magda C.V. Costa-Ribeiro, Vania A. Vicente

Research output: Contribution to journalArticlepeer-review

Abstract

The presence of endophytes promotes the biosynthesis of secondary plant metabolites. In this study, endophytic fungi were isolated from Schinus terebinthifolius to investigate their diversity and antimicrobial activity. A total of 272 endophytic fungi was obtained. These belonged to nine different genera: Alternaria, Colletotrichum, Diaporthe, Epicoccum, Fusarium, Pestalotiopsis, Phyllosticta, Xylaria, and Cryptococcus. Notably, Diaporthe foliorum was introduced as a new species, with accompanying morphological descriptions, illustrations, and a multigene phylogenetic analysis (using ITS, TEF1, TUB, HIS, and CAL). Among the 26 fungal morphotypes evaluated for antimicrobial activity, five strains had inhibitory effects against pathogenic microorganisms. Xylaria allantoidea CMRP1424 extracts showed antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Diaporthe terebinthifolii CMRP1430 and CMRP1436 showed antimicrobial activity against E. coli, P. aeruginosa, S. aureus, and C. albicans. Meanwhile, D. foliorum CMRP1321 and D. malorum CMRP1438 extracts inhibited C. albicans alone. Three classes of chemical compounds were identified in D. foliorum CMRP1438 extracts: ferric chloride, potassium hydroxide, and vanillin–sulfuric acid. In conclusion, the endophytic isolates were able to produce bioactive agents with pharmaceutical potential as antibacterial and antifungal agents. As such, they may provide fresh leads in the search for new, biological sources of drug therapies.

Original languageEnglish (US)
Pages (from-to)3218-3229
Number of pages12
JournalCurrent Microbiology
Volume78
Issue number8
DOIs
StatePublished - Aug 2021

Bibliographical note

Funding Information:
The authors thank the Brazilian agency Coordination for the Improvement of Higher Education—Brazil (CAPES)—Finance Code 001. The work of Santos, G.D., and Gomes, R.R. was supported by Coordination for the Improvement of Higher Education—Brazil (CAPES), and Vicente, V.A. received fellowships from the National Council for Scientific and Technological Development (CNPq). This work was supported by the Brazilian government, the Araucaria Foundation, CAPES, and CNPq.

Funding Information:
This study was supported by the Araucaria Foundation ( http://www.fappr.pr.gov.br/ ), the National Council for Scientific and Technological Development (http:// www.cnpq.br/ ), and the Coordination for the Improvement of Higher Education ( http://www.capes . gov.br/). Institutional Program of Internationalization CAPES/PrInt, Brazil: Grant number 8887.311835/2018–00-AUXPE-2796/2018.

Funding Information:
The authors thank the Brazilian agency Coordination for the Improvement of Higher Education?Brazil (CAPES)?Finance Code 001. The work of Santos, G.D., and Gomes, R.R. was supported by Coordination for the Improvement of Higher Education?Brazil (CAPES), and Vicente, V.A. received fellowships from the National Council for Scientific and Technological Development (CNPq). This work was supported by the Brazilian government, the Araucaria Foundation, CAPES, and CNPq.

Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

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