Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila

Nanako Bowden; Masahiko Takemura; Hiroshi Nakato

doi:10.1007/978-1-0716-1398-6_32

Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila

Nanako Bowden, Masahiko Takemura, Hiroshi Nakato

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila genetics has been a powerful system to explore functions of HSPGs in vivo. In this chapter, we will introduce three genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting), MARCM (mosaic analysis with a repressible cell marker), and FLP-Out.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	405-414
Number of pages	10
Volume	2303
DOIs	https://doi.org/10.1007/978-1-0716-1398-6_32
State	Published - 2022

Publication series

Name	Methods in molecular biology (Clifton, N.J.)
Publisher	Humana Press
ISSN (Print)	1064-3745

Bibliographical note

Funding Information:
We are grateful to Sarah Baker for helpful comments to the manuscript. The authors are supported by National Institutes of Health R35 GM131688 (to H.N.).

Publisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Drosophila melanogaster
FLP-Out
GAL4/UAS
HSPG
MARCM
TARGET

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10.1007/978-1-0716-1398-6_32

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abstract = "Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila genetics has been a powerful system to explore functions of HSPGs in vivo. In this chapter, we will introduce three genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting), MARCM (mosaic analysis with a repressible cell marker), and FLP-Out.",

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author = "Nanako Bowden and Masahiko Takemura and Hiroshi Nakato",

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T1 - Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila

AU - Bowden, Nanako

AU - Takemura, Masahiko

AU - Nakato, Hiroshi

N1 - Funding Information: We are grateful to Sarah Baker for helpful comments to the manuscript. The authors are supported by National Institutes of Health R35 GM131688 (to H.N.). Publisher Copyright: © 2022, Springer Science+Business Media, LLC, part of Springer Nature.

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N2 - Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila genetics has been a powerful system to explore functions of HSPGs in vivo. In this chapter, we will introduce three genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting), MARCM (mosaic analysis with a repressible cell marker), and FLP-Out.

AB - Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila genetics has been a powerful system to explore functions of HSPGs in vivo. In this chapter, we will introduce three genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting), MARCM (mosaic analysis with a repressible cell marker), and FLP-Out.

KW - Drosophila melanogaster

KW - FLP-Out

KW - GAL4/UAS

KW - HSPG

KW - MARCM

KW - TARGET

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M3 - Chapter

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VL - 2303

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila

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