Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila genetics has been a powerful system to explore functions of HSPGs in vivo. In this chapter, we will introduce three genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting), MARCM (mosaic analysis with a repressible cell marker), and FLP-Out.
|Original language||English (US)|
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||10|
|State||Published - 2022|
|Name||Methods in molecular biology (Clifton, N.J.)|
Bibliographical noteFunding Information:
We are grateful to Sarah Baker for helpful comments to the manuscript. The authors are supported by National Institutes of Health R35 GM131688 (to H.N.).
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.
- Drosophila melanogaster