Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila

Nanako Bowden, Masahiko Takemura, Hiroshi Nakato

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila genetics has been a powerful system to explore functions of HSPGs in vivo. In this chapter, we will introduce three genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting), MARCM (mosaic analysis with a repressible cell marker), and FLP-Out.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages405-414
Number of pages10
Volume2303
DOIs
StatePublished - 2022

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
We are grateful to Sarah Baker for helpful comments to the manuscript. The authors are supported by National Institutes of Health R35 GM131688 (to H.N.).

Publisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

  • Drosophila melanogaster
  • FLP-Out
  • GAL4/UAS
  • HSPG
  • MARCM
  • TARGET

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