Molecular genetic changes in human epithelial ovarian malignancies

H. H. Gallion, D. E. Powell, J. K. Morrow, M. Pieretti, E. Case, M. S. Turker, P. D. DePriest, J. E. Hunter, J. R. van Nagell

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

The frequent finding of loss of heterozygosity (LOH) for a specific chromosomal marker in tumor DNA compared to normal DNA suggests the presence of a closely linked tumor-suppressor gene. Using Southern blot analysis, 34 primary ovarian epithelial tumors were examined for the presence of tumor-specific allelic losses, using six probes for chromosomes 6q, 11p, 13q, 16q, and 17p. A high incidence of LOH was observed on 11p, 13q, and 17p. LOH for 17p was present in 3 of 4 (75%) informative benign ovarian tumors, 1 of 5 (20%) borderline tumors, and 16 of 24 (67%) invasive ovarian cancers. Allelic loss with the H-ras1 probe on 11p was present in 10 of 19 (53%) invasive tumors but was not identified in 6 benign or borderline tumors. LOH on 13q was present in 18 of 31 (58%) informative cases including 8 of 10 (80%) Stage 1 tumors. This preliminary study suggests that loss of tumor-suppressor genes on chromosomes 13q and 17p may be early events in ovarian tumorigenesis and that changes on chromosome 11p are later events.

Original languageEnglish (US)
Pages (from-to)137-142
Number of pages6
JournalGynecologic oncology
Volume47
Issue number2
DOIs
StatePublished - Nov 1992

Bibliographical note

Funding Information:
Presented at the 23rd Annual Meeting of the Society of Gynecologic Oncologists, San Antonio, TX, March 15-18, 1992. ’ This research was supported by the following grants: The University of Kentucky Research Fund, GOG Basic Science Grant, and NIH Grant AG 08199.

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