Molecular epidemiology of Types I/III strains of Mycobacterium avium subspecies paratuberculosis isolated from goats and cattle

L. de Juan; J. Álvarez; A. Aranaz; A. Rodríguez; B. Romero; J. Bezos; A. Mateos; L. Domínguez

doi:10.1016/j.vetmic.2006.01.008

Molecular epidemiology of Types I/III strains of Mycobacterium avium subspecies paratuberculosis isolated from goats and cattle

L. de Juan, J. Álvarez, A. Aranaz, A. Rodríguez, B. Romero, J. Bezos, A. Mateos, L. Domínguez

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Molecular characterization of Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis) isolates classifies them into three groups: cattle or Type II, sheep or Type I, and intermediate or Type III. To avoid problems associated with characterization of extremely slow growth strains, PCR-based techniques that divide the M. a. paratuberculosis strains in two main groups (cattle or Type II, and sheep or Types I/III) can be performed. The objectives of this study were to characterize the M. a. paratuberculosis isolates identified by different PCR-based tests (IS1311-PCR and restriction endonuclease analysis, PCR test based on a DNA sequence difference, and a PCR aimed at three Type I-specific loci), and to determine the clinical and epidemiological implications of Types I/III M. a. paratuberculosis strains in livestock. One hundred and fifty-eight M. a. paratuberculosis strains from domestic ruminants were analyzed. One hundred and six M. a. paratuberculosis isolates (61 from goats and 45 from cattle) were classified as Type II strains; and 52 (29 from cows, 20 from goats, and three from sheep) were included in the Types I/III. The Types I/III M. a. paratuberculosis strains were associated to Spanish native breeds. The majority of these animals had not been in direct or indirect contact with sheep flocks infected with M. a. paratuberculosis. This fact should be taken into account when implementing paratuberculosis control programs.

Original language	English (US)
Pages (from-to)	102-110
Number of pages	9
Journal	Veterinary Microbiology
Volume	115
Issue number	1-3
DOIs	https://doi.org/10.1016/j.vetmic.2006.01.008
State	Published - Jun 15 2006
Externally published	Yes

Bibliographical note

Funding Information:
This work was funded by the European Commission (QLRT-2000-01420) and by the Spanish Ministry of Agriculture, Fisheries and Food (MAPA). We thank the collaboration of the Dirección General de Sanidad Agropecuaria, Consejería de Agricultura y Ganadería (Junta de Castilla y León), and the Dirección General de Agricultura, Consejería de Economía e Innovación Tecnológica (Comunidad de Madrid). We are grateful to P. Díez de Tejada, J.M. Fernández, and A. Santos for samples and excellent collaboration. We are grateful to Matthew Gilmour for careful revision of the manuscript.

Keywords

Cattle
Epidemiology
Goat
Molecular
Mycobacterium avium subsp. paratuberculosis
Sheep
Type I
Type II
Type III

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10.1016/j.vetmic.2006.01.008

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title = "Molecular epidemiology of Types I/III strains of Mycobacterium avium subspecies paratuberculosis isolated from goats and cattle",

abstract = "Molecular characterization of Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis) isolates classifies them into three groups: cattle or Type II, sheep or Type I, and intermediate or Type III. To avoid problems associated with characterization of extremely slow growth strains, PCR-based techniques that divide the M. a. paratuberculosis strains in two main groups (cattle or Type II, and sheep or Types I/III) can be performed. The objectives of this study were to characterize the M. a. paratuberculosis isolates identified by different PCR-based tests (IS1311-PCR and restriction endonuclease analysis, PCR test based on a DNA sequence difference, and a PCR aimed at three Type I-specific loci), and to determine the clinical and epidemiological implications of Types I/III M. a. paratuberculosis strains in livestock. One hundred and fifty-eight M. a. paratuberculosis strains from domestic ruminants were analyzed. One hundred and six M. a. paratuberculosis isolates (61 from goats and 45 from cattle) were classified as Type II strains; and 52 (29 from cows, 20 from goats, and three from sheep) were included in the Types I/III. The Types I/III M. a. paratuberculosis strains were associated to Spanish native breeds. The majority of these animals had not been in direct or indirect contact with sheep flocks infected with M. a. paratuberculosis. This fact should be taken into account when implementing paratuberculosis control programs.",

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author = "{de Juan}, L. and J. {\'A}lvarez and A. Aranaz and A. Rodr{\'i}guez and B. Romero and J. Bezos and A. Mateos and L. Dom{\'i}nguez",

note = "Funding Information: This work was funded by the European Commission (QLRT-2000-01420) and by the Spanish Ministry of Agriculture, Fisheries and Food (MAPA). We thank the collaboration of the Direcci{\'o}n General de Sanidad Agropecuaria, Consejer{\'i}a de Agricultura y Ganader{\'i}a (Junta de Castilla y Le{\'o}n), and the Direcci{\'o}n General de Agricultura, Consejer{\'i}a de Econom{\'i}a e Innovaci{\'o}n Tecnol{\'o}gica (Comunidad de Madrid). We are grateful to P. D{\'i}ez de Tejada, J.M. Fern{\'a}ndez, and A. Santos for samples and excellent collaboration. We are grateful to Matthew Gilmour for careful revision of the manuscript.",

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AU - Rodríguez, A.

AU - Romero, B.

AU - Bezos, J.

AU - Mateos, A.

AU - Domínguez, L.

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Molecular epidemiology of Types I/III strains of Mycobacterium avium subspecies paratuberculosis isolated from goats and cattle

Abstract

Bibliographical note

Keywords

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