TY - JOUR
T1 - Molecular epidemiology of multidrug-resistant Mycobacterium bovis isolates with the same spoligotyping profile as isolates from animals
AU - Romero, Beatriz
AU - Aranaz, Alicia
AU - De Juan, Lucía
AU - Álvarez, Julio
AU - Bezos, Javier
AU - Mateos, Ana
AU - Gómez-Mampaso, Enrique
AU - Domínguez, Lucas
PY - 2006/9
Y1 - 2006/9
N2 - PCR-based characterization techniques have been adopted in most laboratories for Mycobacterium bovis typing. We report a molecular characterization of human multidrug-resistant M. bovis isolates and three bovine isolates that share the spoligotyping profile. The analysis of the direct repeat region showed that both groups differed in the presence of spacers not included in the current membrane. They were also distinguished by two out of the nine mycobacterial interspersed repetitive unit variable-number tandem repeat loci tested, indicating that the human infection was not acquired from the cattle from which isolates were obtained. These results highlight that a combination of techniques is required for appropriate discrimination, even for those spoligotypes that have a low frequency.
AB - PCR-based characterization techniques have been adopted in most laboratories for Mycobacterium bovis typing. We report a molecular characterization of human multidrug-resistant M. bovis isolates and three bovine isolates that share the spoligotyping profile. The analysis of the direct repeat region showed that both groups differed in the presence of spacers not included in the current membrane. They were also distinguished by two out of the nine mycobacterial interspersed repetitive unit variable-number tandem repeat loci tested, indicating that the human infection was not acquired from the cattle from which isolates were obtained. These results highlight that a combination of techniques is required for appropriate discrimination, even for those spoligotypes that have a low frequency.
UR - http://www.scopus.com/inward/record.url?scp=33748774423&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748774423&partnerID=8YFLogxK
U2 - 10.1128/JCM.00730-06
DO - 10.1128/JCM.00730-06
M3 - Article
C2 - 16954286
AN - SCOPUS:33748774423
SN - 0095-1137
VL - 44
SP - 3405
EP - 3408
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 9
ER -