Molecular dynamics of Emiliania huxleyi and cooccurring viruses during two separate mesocosm studies

Joaquín Martínez Martínez, Declan C. Schroeder, Aud Larsen, Gunnar Bratbak, William H. Wilson

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

In this study we used denaturing gradient gel electrophoresis, sequencing analysis, and analytical flow cytometry to monitor the dynamics and genetic richness of Emillania huxleyi isolates and cooccurring viruses during two mesocosm experiments in a Norwegian fjord in 2000 and 2003. We exploited variations in a gene encoding a protein with calcium-binding motifs (GPA) and in the major capsid protein (MCP) gene to assess allelic and genotypic richness within E. huxleyi and E. huxleyi-specific viruses (EhVs), respectively. To our knowledge, this is the first report that shows the effectiveness of the GPA gene for analysis of natural communities of E. huxleyi. Our results revealed the existence of a genetically rich, yet stable E. huxleyi and EhV community in the fjordic environment. Incredibly, the same virus and host genotypes dominated in separate studies conducted 3 years apart. Both E. huxleyi-dominated blooms contained the same six E. huxleyi alleles. In addition, despite the presence of at least six and four EhV genotypes at the start of the blooms in 2000 and 2003, respectively, the same two virus genotypes dominated the naturally occurring infections during the exponential and termination phases of the blooms in both years.

Original languageEnglish (US)
Pages (from-to)554-562
Number of pages9
JournalApplied and environmental microbiology
Volume73
Issue number2
DOIs
StatePublished - Jan 2007

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