Abstract
A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences of Labeo rohita, Cirrhina mrigala and Catla catla have been cloned, characterized and overexpressed in Escherichia coli. These sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 261-272 |
| Number of pages | 12 |
| Journal | Journal of Biosciences |
| Volume | 27 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2002 |
| Externally published | Yes |
Keywords
- Growth hormone
- Indian major carps
- Overexpression
- Protein 3-D structure
- RACE
- cDNA cloning