Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy

T. Venugopal, S. Mathavan, T. J. Pandian

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences of Labeo rohita, Cirrhina mrigala and Catla catla have been cloned, characterized and overexpressed in Escherichia coli. These sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.

Original languageEnglish (US)
Pages (from-to)261-272
Number of pages12
JournalJournal of Biosciences
Volume27
Issue number3
DOIs
StatePublished - Jan 1 2002

Keywords

  • Growth hormone
  • Indian major carps
  • Overexpression
  • Protein 3-D structure
  • RACE
  • cDNA cloning

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