Molecular cloning of canine membrane-anchored inhibitor of matrix metalloproteinase, RECK

Satoshi Takagi, Takanori Kitamura, Yoshinao Hosaka, Tomohiro Ohsaki, Darko Bosnakovski, Tsuyoshi Kadosawa, Masahiro Okumura, Toru Fujinaga

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The reversion-inducing cysteine-rich protein with Kazal motifs (RECK) gene is one of the endogenous matrix metalloproteinase (MMP) inhibitors. It was reported that decreased RECK expression closely correlated with tumor malignancy. We determined the cDNA sequence of the canine RECK gene. The cDNA sequence and deduced amino acid of canine RECK were 2,913 bases and 971 residues, respectively. The predicted amino acid sequence of the protein showed 95.5% and 91.9% homology with human and mouse RECK, respectively. RECK mRNA expression was analyzed in various canine tissues and tumor cell lines by quantitative RT-PCR. The highest RECK expression was detected in lung and testis. In comparison with the tissues, a remarkably low expression level was detected in tumor cell lines. In addition, the RECK gene was transfected in the canine transitional cell carcinoma, and its influence on cell proliferation, migration, and invasion was analyzed. The transfected RECK gene suppressed only canine tumor invasion. These results showed that RECK might play an important role in tumor malignancy in dogs as well as in other mammalians.

Original languageEnglish (US)
Pages (from-to)385-391
Number of pages7
JournalJournal of Veterinary Medical Science
Volume67
Issue number4
DOIs
StatePublished - Apr 2005
Externally publishedYes

Keywords

  • Canine
  • Extracellular matrix
  • Proteinase inhibitor
  • RECK
  • Tumor malignancy

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