Molecular cloning of a novel protein regulated by opioid treatment of NG108-15 cells

Marilee J. Wick; David K. Ann; Horace H Loh

doi:10.1016/0169-328X(95)00090-F

Molecular cloning of a novel protein regulated by opioid treatment of NG108-15 cells

Marilee J. Wick, David K. Ann, Horace H Loh

Pharmacology

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

A new cDNA clone, NGD5, has been identified from a subtraction cDNA library constructed with mRNA isolated from control neuroblastoma X glioma NG108-15 cells and cells treated for 48 h with the δ-opioid agonist, d-Ala², d-Leu⁵ enkephalin (DADLE). NGD5 mRNA is decreased, in a naloxone-reversible manner, upon long-term treatment of NG108-15 cells with DADLE, indicating that this clone may be related to opioid receptor function. Northern analysis indicates that NGD5 mRNA is expressed in rat brain. Two similar nearly full-length NGD5 clones, NGD5A and NGD5B, were isolated from a λgt10 NG108-15 cDNA library and sequenced. The predicted 40-kDa peptide encoded for by the NGD5 cDNA has no significant homology to the recently cloned μ δ or κ opioid receptors nor to any other known proteins.

Original language	English (US)
Pages (from-to)	171-175
Number of pages	5
Journal	Molecular Brain Research
Volume	32
Issue number	1
DOIs	https://doi.org/10.1016/0169-328X(95)00090-F
State	Published - Aug 1995

Bibliographical note

Funding Information:
This research was supported by NIH Grants DA 00564, DA 01583, DA 05695, K05-DA-70554 and the F and A Stark Fund of the Minnesota Medical Foundation.

Keywords

Down regulation
Neuroblastoma cell
Subtractive cDNA cloning
δ-Opioid receptor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Publisher link

10.1016/0169-328X(95)00090-F

Find It

Find It at U of M Libraries

Cite this

@article{e9475cdc1d4944d8be145569d4d33815,

title = "Molecular cloning of a novel protein regulated by opioid treatment of NG108-15 cells",

abstract = "A new cDNA clone, NGD5, has been identified from a subtraction cDNA library constructed with mRNA isolated from control neuroblastoma X glioma NG108-15 cells and cells treated for 48 h with the δ-opioid agonist, d-Ala2, d-Leu5 enkephalin (DADLE). NGD5 mRNA is decreased, in a naloxone-reversible manner, upon long-term treatment of NG108-15 cells with DADLE, indicating that this clone may be related to opioid receptor function. Northern analysis indicates that NGD5 mRNA is expressed in rat brain. Two similar nearly full-length NGD5 clones, NGD5A and NGD5B, were isolated from a λgt10 NG108-15 cDNA library and sequenced. The predicted 40-kDa peptide encoded for by the NGD5 cDNA has no significant homology to the recently cloned μ δ or κ opioid receptors nor to any other known proteins.",

keywords = "Down regulation, Neuroblastoma cell, Subtractive cDNA cloning, δ-Opioid receptor",

author = "Wick, \{Marilee J.\} and Ann, \{David K.\} and Loh, \{Horace H\}",

note = "Funding Information: This research was supported by NIH Grants DA 00564, DA 01583, DA 05695, K05-DA-70554 and the F and A Stark Fund of the Minnesota Medical Foundation.",

year = "1995",

month = aug,

doi = "10.1016/0169-328X(95)00090-F",

language = "English (US)",

volume = "32",

pages = "171--175",

journal = "Molecular Brain Research",

issn = "0169-328X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Molecular cloning of a novel protein regulated by opioid treatment of NG108-15 cells

AU - Wick, Marilee J.

AU - Ann, David K.

AU - Loh, Horace H

N1 - Funding Information: This research was supported by NIH Grants DA 00564, DA 01583, DA 05695, K05-DA-70554 and the F and A Stark Fund of the Minnesota Medical Foundation.

PY - 1995/8

Y1 - 1995/8

N2 - A new cDNA clone, NGD5, has been identified from a subtraction cDNA library constructed with mRNA isolated from control neuroblastoma X glioma NG108-15 cells and cells treated for 48 h with the δ-opioid agonist, d-Ala2, d-Leu5 enkephalin (DADLE). NGD5 mRNA is decreased, in a naloxone-reversible manner, upon long-term treatment of NG108-15 cells with DADLE, indicating that this clone may be related to opioid receptor function. Northern analysis indicates that NGD5 mRNA is expressed in rat brain. Two similar nearly full-length NGD5 clones, NGD5A and NGD5B, were isolated from a λgt10 NG108-15 cDNA library and sequenced. The predicted 40-kDa peptide encoded for by the NGD5 cDNA has no significant homology to the recently cloned μ δ or κ opioid receptors nor to any other known proteins.

AB - A new cDNA clone, NGD5, has been identified from a subtraction cDNA library constructed with mRNA isolated from control neuroblastoma X glioma NG108-15 cells and cells treated for 48 h with the δ-opioid agonist, d-Ala2, d-Leu5 enkephalin (DADLE). NGD5 mRNA is decreased, in a naloxone-reversible manner, upon long-term treatment of NG108-15 cells with DADLE, indicating that this clone may be related to opioid receptor function. Northern analysis indicates that NGD5 mRNA is expressed in rat brain. Two similar nearly full-length NGD5 clones, NGD5A and NGD5B, were isolated from a λgt10 NG108-15 cDNA library and sequenced. The predicted 40-kDa peptide encoded for by the NGD5 cDNA has no significant homology to the recently cloned μ δ or κ opioid receptors nor to any other known proteins.

KW - Down regulation

KW - Neuroblastoma cell

KW - Subtractive cDNA cloning

KW - δ-Opioid receptor

UR - https://www.scopus.com/pages/publications/0029048008

UR - https://www.scopus.com/inward/citedby.url?scp=0029048008&partnerID=8YFLogxK

U2 - 10.1016/0169-328X(95)00090-F

DO - 10.1016/0169-328X(95)00090-F

M3 - Article

C2 - 7494458

AN - SCOPUS:0029048008

SN - 0169-328X

VL - 32

SP - 171

EP - 175

JO - Molecular Brain Research

JF - Molecular Brain Research

IS - 1

ER -

Molecular cloning of a novel protein regulated by opioid treatment of NG108-15 cells

Abstract

Bibliographical note

Keywords

UN SDGs

Publisher link

Find It

Other files and links

Fingerprint

Cite this