Molecular cloning of a novel protein regulated by opioid treatment of NG108-15 cells

Marilee J. Wick, David K. Ann, Horace H Loh

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

A new cDNA clone, NGD5, has been identified from a subtraction cDNA library constructed with mRNA isolated from control neuroblastoma X glioma NG108-15 cells and cells treated for 48 h with the δ-opioid agonist, d-Ala2, d-Leu5 enkephalin (DADLE). NGD5 mRNA is decreased, in a naloxone-reversible manner, upon long-term treatment of NG108-15 cells with DADLE, indicating that this clone may be related to opioid receptor function. Northern analysis indicates that NGD5 mRNA is expressed in rat brain. Two similar nearly full-length NGD5 clones, NGD5A and NGD5B, were isolated from a λgt10 NG108-15 cDNA library and sequenced. The predicted 40-kDa peptide encoded for by the NGD5 cDNA has no significant homology to the recently cloned μ δ or κ opioid receptors nor to any other known proteins.

Original languageEnglish (US)
Pages (from-to)171-175
Number of pages5
JournalMolecular Brain Research
Volume32
Issue number1
DOIs
StatePublished - Aug 1995

Keywords

  • Down regulation
  • Neuroblastoma cell
  • Subtractive cDNA cloning
  • δ-Opioid receptor

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