Molecular cloning of a human histocompatibility antigen cDNA fragment

H. L. Ploegh, H. T. Orr, J. L. Strominger

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85 Scopus citations


A clone (pHLA-1) containing HLA-specific cDNA was constructed by reverse transcription of partially purified HLA mRNA from the human lymphoblastoid cell line LKT. The identity of pHLA-1 was established by its ability to hybridize to HLA heavy chain mRNA and by nucleotide sequence analysis. The pHLA-1 cDNA insert (~525 base pairs) corresponds to the COOH-terminal 46 amino acids of an HLA-A, -B, or -C antigen (15 residues from the hydrophobic region and the remainder from the COOH-terminal hydrophilic region), together with a portion of the 3' terminal untranslated region of the mRNA.

Original languageEnglish (US)
Pages (from-to)6081-6085
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number10 II
StatePublished - 1980


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