Molecular cloning, functional characterization and expression analysis of a novel monosaccharide transporter gene OsMST6 from rice (Oryza sativa L.)

Yongqin Wang, Yuguo Xiao, Yu Zhang, Chenglin Chai, Gang Wei, Xiaoli Wei, Honglin Xu, Mei Wang, Pieter B.F. Ouwerkerk, Zhen Zhu

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Monosaccharides transporters play important roles in assimilate supply for sink tissue development. In this study, a new monosaccharide transporter gene OsMST6 was identified from rice (Oryza sativa L.). The predicted OsMST6 protein shows typical features of sugar transporters and shares 79.6% identity with the rice monosaccharide transporter OsMST3. Heterologous expression in yeast (Saccharomyces cerevisiae) demonstrated that OsMST6 is a broad-spectrum monosaccharide transporter, with a K m of 266.1 μΜ for glucose. OsMST6-green fluorescent protein fusion protein is localized to the plasma membrane in plant. Semi-quantitative RT-PCR analysis exhibited that OsMST6 is expressed in all tested organs/tissues. In developing seeds, OsMST6 expression level is high at the early and middle grain filling stages and gradually declines later. Further analysis detected its expression in both maternal and filial tissues. RNA in situ hybridization analysis indicated that OsMST6 is predominantly expressed in the vascular parenchyma of the chalazal vein, cross-cells, nucellar tissue and endosperm of young seeds, in mesophyll cells of source leaf blades, and in pollens and the connective vein of anthers. In addition, OsMST6 expression is up-regulated by salt stress and sugars. The physiological role of OsMST6 for seed development and its roles in other sink and source tissues are discussed.

Original languageEnglish (US)
Pages (from-to)525-535
Number of pages11
JournalPlanta
Volume228
Issue number4
DOIs
StatePublished - Sep 2008

Keywords

  • Expression pattern
  • Monosaccharide transporter
  • Oryza
  • Seed development
  • cDNA cloning

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