Molecular cloning, expression, and purification of nuclear inclusion a protease from tobacco vein mottling virus

Duk Chul Hwang, Do Hyung Kim, Byoung Heon Kang, Byeong Doo Song, Kwan Yong Choi

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The gene encoding the C-terminal protease domain of the nuclear inclusion protein a (NIa) of tobacco vein mottling virus (TVMV) was cloned from an isolated virus particle and expressed as a fusion protein with glutathione S-transferase in Escherichia coli XL1-blue. The 27-kDa protease was purified from the fusion protein by glutathione affinity chromatography and Mono S chromatography. The purified protease exhibited the specific proteolytic activity towards the nonapeptide substrates, Ac-Glu-Asn-Asn-Val-Arg-Phe-Gln-Ser-Leu-amide and Ac-Arg-Glu-Thr-Val-Arg-Phe-Gln-Ser-Asp-amide, containing the junction sequences between P3 protein and cylindrical inclusion protein and between nuclear inclusion protein b and capsid protein, respectively. The Km and kcat values were about 0.2 mM and 0.071 s-1, respectively, which were approximately five-fold lower than those obtained for the NIa protease of turnip mosaic potyvirus (TuMV), suggesting that the TVMV NIa protease is different in the binding affinity as well as in the catalytic power from the TuMV NIa protease. In contrast to the NIa proteases from TuMV and tobacco etch virus, the TVMV NIa protease was not autocatalytically cleaved into smaller proteins, indicating that the C-terminal truncation is not a common phenomenon occurring in all potyviral NIa proteases. These results suggest that the TVMV NIa protease has a unique biochemical property distinct from those of other potyviral proteases.

Original languageEnglish (US)
Pages (from-to)148-155
Number of pages8
JournalMolecules and cells
Issue number2
StatePublished - Apr 30 2000

Bibliographical note

Funding Information:
Acknowledgments This work was supported partly by a grant from the Genetic Engineering Program from Ministry of Education (BDS) and by the Agricultural R & D Promotion Center.


  • Nuclear Inclusion Protein A
  • Potyvirus
  • TVMV
  • Viral Protease


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