Molecular cloning and structural analysis of canine gastric H+, K+-ATPase

Il Song, M. Patricia Mortell, Ira Gantz, David R. Brown, Tadataka Yamada

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Gastric hydrogen-potassium ATPase (H+, K+-ATPase) is a heterodimeric protein which participates in the formation of hydrochloric acid. We cloned canine H+, K+-ATPase αand β subunit cDNAs from canine gastric cDNA libraries and the αsubunit gene from a canine genomic library. The αsubunit gene is 13 kb in length and contains 21 introns ranging from 77 to 1,076 bp. Its 5ߣ-flanking region contains putative regulatory motifs for transcription that are similar to those found in H+, K+-ATPase genes from other species. The open reading frames of αand β subunit cDNAs are 3,500 and 870 bp in length and encode proteins of 1,034 and 290 amino acids, respectively. They are 80 - 90% homologous to corresponding cDNAs previously identified in porcine and rodent gastric tissues.

Original languageEnglish (US)
Pages (from-to)1240-1247
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Nov 15 1993


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