Molecular cloning and nucleotide sequence analysis of cholera toxin genes of the CtxA- Vibrio cholerae strain Texas Star-SR

T. J. Brickman, M. Boesman-Finkelstein, R. A. Finkelstein, M. A. McIntosh

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The ctx operons from the Vibrio cholerae El Tor strain 3083 and its CtxA- derivative Texas Star-SR were cloned, and their nucleotide sequences were compared. A single missense mutation in the Texas Star-SR ctxA cistron which results in the substitution of threonine for alanine at position 191 of the 258-amino-acid CtxA precursor was identified. Immunoblot analysis detected the mutant CtxA intracellularly early in the culture cycle but not extracellularly at any growth stage.

Original languageEnglish (US)
Pages (from-to)4142-4144
Number of pages3
JournalInfection and immunity
Volume58
Issue number12
DOIs
StatePublished - 1990

Fingerprint Dive into the research topics of 'Molecular cloning and nucleotide sequence analysis of cholera toxin genes of the CtxA<sup>-</sup> Vibrio cholerae strain Texas Star-SR'. Together they form a unique fingerprint.

Cite this