Abstract
The ctx operons from the Vibrio cholerae El Tor strain 3083 and its CtxA- derivative Texas Star-SR were cloned, and their nucleotide sequences were compared. A single missense mutation in the Texas Star-SR ctxA cistron which results in the substitution of threonine for alanine at position 191 of the 258-amino-acid CtxA precursor was identified. Immunoblot analysis detected the mutant CtxA intracellularly early in the culture cycle but not extracellularly at any growth stage.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4142-4144 |
| Number of pages | 3 |
| Journal | Infection and immunity |
| Volume | 58 |
| Issue number | 12 |
| DOIs | |
| State | Published - 1990 |
