The keratinocyte lipid-binding protein (KLBP or E-FABP) is a member of a large family of intracelluîar fatty acid-binding proteins. Because of its unique tissue distribution (skin and tongue epithelia, adipose, mammary, lens and endothelial cells) and metabolic responsiveness (upregulated in skin carcinoma, psoriasis and crushed spinal cord neurons) we have undertaken an analysis of the KLBP gene and its regulation. Southern analysis using a partial cDNA probe revealed the presence of multiple bands suggesting several genes or pseudogenes in the mouse genome. Based upon the highly conserved structure of lipidbinding protein genes, the third intron from the putative authentically expressed KLBP gene was amplified via PCR and used to identify one unique gene which contained the classical four exon-three intron motif characteristic of all other cloned lipid-binding prolein genes. A functional promoter for the KLBP gene was demonstrated by transient transfections of a KLBP-luciferase reporter gene into JB6 keratinocytes cells. Using the genomic fragment, fluorescence in-situ hybridization identified the location of the KLBP gene cytogenetically to the end of chromosome It. Therefore, the KLBP gene, like that for the adipocyte lipid-binding protein gene (aP2). myeliri P2 gene, and intestinal fatty acidbinding protein gene, all reside on chromosome 3.
|Original language||English (US)|
|State||Published - Dec 1 1998|