Molecular cloning and characterization of a multidrug efflux pump, SmfY, from Serratia marcescens

Fereshteh Shahcheraghi; Yusuke Minato; Jing Chen; Tohru Mizushima; Wakano Ogawa; Teruo Kuroda; Tomofusa Tsuchiya

doi:10.1248/bpb.30.798

Molecular cloning and characterization of a multidrug efflux pump, SmfY, from Serratia marcescens

Fereshteh Shahcheraghi, Yusuke Minato, Jing Chen, Tohru Mizushima, Wakano Ogawa, Teruo Kuroda, Tomofusa Tsuchiya

Microbiology

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23 Scopus citations

Abstract

We cloned a gene smfY for multidrug efflux pump from chromosomal DNA of Serratia marcescens using drug-hypersensitive Escherichia coli KAM32 as the host, and characterized the pump. E. coli KAM32/pESM42 carrying the smfY showed significantly increased MICs of various drugs including DAPI, norfloxacin, benzalkonium chloride, acriflavine and ethidium bromide, compared with the control. We also detected energy-dependent ethidium and acriflavine efflux due to the SmfY. Sequence analysis revealed that the SmfY was a multidrug ef-flux pump of the MF (Major Facilitator) superfamily transporters. This is the first report of a multidrug efflux pump belonging to the MF superfamily in S. marcescens.

Original language	English (US)
Pages (from-to)	798-800
Number of pages	3
Journal	Biological and Pharmaceutical Bulletin
Volume	30
Issue number	4
DOIs	https://doi.org/10.1248/bpb.30.798
State	Published - Apr 2007

Keywords

Major facilitator (MF) superfamily
Multidrug efflux pump
Serratia marcescens
SmfY

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10.1248/bpb.30.798

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abstract = "We cloned a gene smfY for multidrug efflux pump from chromosomal DNA of Serratia marcescens using drug-hypersensitive Escherichia coli KAM32 as the host, and characterized the pump. E. coli KAM32/pESM42 carrying the smfY showed significantly increased MICs of various drugs including DAPI, norfloxacin, benzalkonium chloride, acriflavine and ethidium bromide, compared with the control. We also detected energy-dependent ethidium and acriflavine efflux due to the SmfY. Sequence analysis revealed that the SmfY was a multidrug ef-flux pump of the MF (Major Facilitator) superfamily transporters. This is the first report of a multidrug efflux pump belonging to the MF superfamily in S. marcescens.",

keywords = "Major facilitator (MF) superfamily, Multidrug efflux pump, Serratia marcescens, SmfY",

author = "Fereshteh Shahcheraghi and Yusuke Minato and Jing Chen and Tohru Mizushima and Wakano Ogawa and Teruo Kuroda and Tomofusa Tsuchiya",

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AU - Shahcheraghi, Fereshteh

AU - Minato, Yusuke

AU - Chen, Jing

AU - Mizushima, Tohru

AU - Ogawa, Wakano

AU - Kuroda, Teruo

AU - Tsuchiya, Tomofusa

PY - 2007/4

Y1 - 2007/4

N2 - We cloned a gene smfY for multidrug efflux pump from chromosomal DNA of Serratia marcescens using drug-hypersensitive Escherichia coli KAM32 as the host, and characterized the pump. E. coli KAM32/pESM42 carrying the smfY showed significantly increased MICs of various drugs including DAPI, norfloxacin, benzalkonium chloride, acriflavine and ethidium bromide, compared with the control. We also detected energy-dependent ethidium and acriflavine efflux due to the SmfY. Sequence analysis revealed that the SmfY was a multidrug ef-flux pump of the MF (Major Facilitator) superfamily transporters. This is the first report of a multidrug efflux pump belonging to the MF superfamily in S. marcescens.

AB - We cloned a gene smfY for multidrug efflux pump from chromosomal DNA of Serratia marcescens using drug-hypersensitive Escherichia coli KAM32 as the host, and characterized the pump. E. coli KAM32/pESM42 carrying the smfY showed significantly increased MICs of various drugs including DAPI, norfloxacin, benzalkonium chloride, acriflavine and ethidium bromide, compared with the control. We also detected energy-dependent ethidium and acriflavine efflux due to the SmfY. Sequence analysis revealed that the SmfY was a multidrug ef-flux pump of the MF (Major Facilitator) superfamily transporters. This is the first report of a multidrug efflux pump belonging to the MF superfamily in S. marcescens.

KW - Major facilitator (MF) superfamily

KW - Multidrug efflux pump

KW - Serratia marcescens

KW - SmfY

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IS - 4

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Molecular cloning and characterization of a multidrug efflux pump, SmfY, from Serratia marcescens

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