Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori

Hiroshi Sawada, Hiroshi Nakato, Toru Togawa, Motoko Nakagoshi, Shin Ichiro Takikawa, Kenjiro Dohke, Teruhiko Iino, Keisuke Mase, Toshio Yamamoto, Susumu Izumi

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We have cloned the full length of a novel cDNA named Bombyx mori cuticle protein that contains an AlaAlaProAla/Val-repeat (BMCPA) from a cDNA library of integument in the larval silkworm. Both a typical tandem repeat (A-A-P-A/V) for cuticle protein and a unique tandem repeat with Ser, Ala, Gly, Pro, Val, Tyr and Thr were observed in the predicted amino acid sequence of the cDNA encoding BMCPA. Approximately 80% of the amino acids in BMCPA were composed of Ser, Ala, Gly, Pro, Val and Tyr. Northern-hybridization analysis indicated that BMCPA mRNA is expressed only in the larval epidermis and that the expression pattern of the BMCPA gene in the developmental stage was observed mainly at the larval stage. We propose BMCPA may be a novel component of cuticle, and may play an important role in the integument of the larval silkworm.

Original languageEnglish (US)
Pages (from-to)519-527
Number of pages9
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume134
Issue number3
DOIs
StatePublished - Mar 1 2003

Keywords

  • Alanine-alanine-proline/valine-repeat
  • Bombyx mori
  • Cuticle protein
  • Gene expression
  • Primary structure
  • R&R consensus
  • RNA polymerase II
  • cDNA cloning

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