Molecular characterization of the newly identified human parvovirus 4 in the family Parvoviridae

Sai Lou, Baoyan Xu, Qinfeng Huang, Ning Zhi, Fang Cheng, Susan Wong, Kevin Brown, Eric Delwart, Zhengwen Liu, Jianming Qiu

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Human parvovirus 4 (PARV4) is an emerging human virus, and little is known about the molecular aspects of PARV4 apart from its incomplete genome sequence, which lacks information of the termini. We analyzed the gene expression profile of PARV4 using a nearly full-length HPV4 genome in a replication competent system in 293 cells. We found that PARV4 utilizes two promoters to transcribe non-structural protein- and structural protein-encoding mRNAs, respectively, which were polyadenylated at the right end of the genome. Three major proteins, including the large non-structural protein NS1a, whose mRNA is spliced, and capsid proteins VP1 and VP2, were detected. Additional functional analysis of the NS1a revealed its capability to induce cell cycle arrest at G2/M phase in ex vivo-generated human hematopoietic stem cells. Taken together, our characterization of the molecular features of PARV4 suggests that PARV4 represents a new genus in the family Parvoviridae.

Original languageEnglish (US)
Pages (from-to)59-69
Number of pages11
JournalVirology
Volume422
Issue number1
DOIs
StatePublished - Jan 5 2012

Bibliographical note

Funding Information:
This work was supported by PHS grant R01 AI070723 from NIAID to JQ . We thank the members of Qiu lab for their valuable discussions.

Keywords

  • Gene expression
  • Parvovirus

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