Molecular characterization of a novel cell surface ADP-ribosyl cyclase from the sea urchin

Dev Churamani, Michael J. Boulware, Latha Ramakrishnan, Timothy J. Geach, Andrew C R Martin, Victor D. Vacquier, Jonathan S. Marchant, Leslie Dale, Sandip Patel

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The sea urchin is an extensively used model system for the study of calcium signalling by the messenger molecules NAADP and cyclic ADP-ribose. Both are synthesized by ADP-ribosyl cyclases but our molecular understanding of these enzymes in the sea urchin is limited. We have recently reported the cloning of an extended family of sea urchin ADP-ribosyl cyclases and shown that one of these enzymes (SpARC1) is active within the endoplasmic reticulum lumen. These studies suggest that production of messengers is compartmentalized. Here we characterize the properties of SpARC2. SpARC2 catalyzed both NAADP and cyclic ADP-ribose production. Unusually, the NAD surrogate, NGD was a poor substrate. In contrast to SpARC1, heterologously expressed SpARC2 localized to the plasma membrane via a glycosylphosphatidylinositol (GPI)-anchor. Transcripts for SpARC2 were readily detectable in sea urchin eggs and a majority of the endogenous membrane bound activity was found to be GPI-anchored. Our data reveal striking differences in the properties of sea urchin ADP-ribosyl cyclases and provide further evidence that messenger production may occur outside of the cytosol.

Original languageEnglish (US)
Pages (from-to)2347-2355
Number of pages9
JournalCellular Signalling
Volume20
Issue number12
DOIs
StatePublished - Dec 1 2008

Fingerprint

ADP-ribosyl Cyclase
Sea Urchins
Cyclic ADP-Ribose
Glycosylphosphatidylinositols
Calcium Signaling
Enzymes
Endoplasmic Reticulum
NAD
Cytosol
Eggs
Organism Cloning
Cell Membrane
Membranes

Keywords

  • ADP-ribosyl cyclases
  • Cyclic ADP-ribose
  • NAADP
  • Sea urchin

Cite this

Churamani, D., Boulware, M. J., Ramakrishnan, L., Geach, T. J., Martin, A. C. R., Vacquier, V. D., ... Patel, S. (2008). Molecular characterization of a novel cell surface ADP-ribosyl cyclase from the sea urchin. Cellular Signalling, 20(12), 2347-2355. https://doi.org/10.1016/j.cellsig.2008.09.005

Molecular characterization of a novel cell surface ADP-ribosyl cyclase from the sea urchin. / Churamani, Dev; Boulware, Michael J.; Ramakrishnan, Latha; Geach, Timothy J.; Martin, Andrew C R; Vacquier, Victor D.; Marchant, Jonathan S.; Dale, Leslie; Patel, Sandip.

In: Cellular Signalling, Vol. 20, No. 12, 01.12.2008, p. 2347-2355.

Research output: Contribution to journalArticle

Churamani, D, Boulware, MJ, Ramakrishnan, L, Geach, TJ, Martin, ACR, Vacquier, VD, Marchant, JS, Dale, L & Patel, S 2008, 'Molecular characterization of a novel cell surface ADP-ribosyl cyclase from the sea urchin', Cellular Signalling, vol. 20, no. 12, pp. 2347-2355. https://doi.org/10.1016/j.cellsig.2008.09.005
Churamani D, Boulware MJ, Ramakrishnan L, Geach TJ, Martin ACR, Vacquier VD et al. Molecular characterization of a novel cell surface ADP-ribosyl cyclase from the sea urchin. Cellular Signalling. 2008 Dec 1;20(12):2347-2355. https://doi.org/10.1016/j.cellsig.2008.09.005
Churamani, Dev ; Boulware, Michael J. ; Ramakrishnan, Latha ; Geach, Timothy J. ; Martin, Andrew C R ; Vacquier, Victor D. ; Marchant, Jonathan S. ; Dale, Leslie ; Patel, Sandip. / Molecular characterization of a novel cell surface ADP-ribosyl cyclase from the sea urchin. In: Cellular Signalling. 2008 ; Vol. 20, No. 12. pp. 2347-2355.
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