Molecular characterization of a novel Camelus dromedarius papillomavirus

Nader M. Sobhy, Vikash Singh, Hend M.El Damaty, Sunil K. Mor, Christiana R.B. Youssef, Sagar M. Goyal

Research output: Contribution to journalArticlepeer-review

Abstract

Papillomaviruses affect both human and non-human hosts. In camels, papillomatosis is caused by Camelus dromedarius papillomavirus type 1 and 2 (CdPV1 and CdPV2, respectively). In late 2018, an outbreak of camelpox occurred in a herd of fattening camels in Egypt. Several animals were found to be co-infected with camelpox and camel papillomaviruses. The morbidity with papillomatosis was 35 %. The infection was confirmed by PCR then Illumina sequencing revealed the presence of a complete genome of two CdPVs. One of these was CdPV1 (MT130101) and the other was a putative novel virus, tentatively named as CdPV3 (MT130100). Seven ORFs and a long upstream regulatory region were identified in the genomes of both viruses. Pairwise comparisons of L1 gene revealed 98.92 % nt identity between MT130101/CdPV1/Egypt/2018 and HQ912790/CdPV1/Sudan/2009 with 100 % coverage. However, MT130100/CdPV3/ Egypt/2018 showed only 68.99 % nt identity with the closest genome HQ912791/CdPV2/Sudan/2009. Phylogenetic analyses indicated that CdPV1 and CdPV3 belonged to the genus Deltapapillomavirus. These results should be useful for future CdPVs molecular surveillance and construction of evolutionary characteristics of this virus.

Original languageEnglish (US)
Article number101561
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume73
DOIs
StatePublished - Dec 2020

Keywords

  • Camels
  • Camelus dromedarius
  • Egypt
  • Next-generation sequencing
  • Papillomaviruses

PubMed: MeSH publication types

  • Journal Article

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