Molecular characterization and pathogenicity of a carrot (Daucus carota) infecting begomovirus and associated betasatellite from India

Jitendra Kumar, Samatha Gunapati, Sudhir P. Singh, Rekha Gadre, Naresh C. Sharma, Rakesh Tuli

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The yellow mosaic pattern and shortening of leaf petiole are common disease symptoms associated with begomovirus infection in carrot. DNA from field infected carrot leaves was analyzed by rolling circle amplification and sequencing. The results established the presence of ageratum enation virus (AEV), which is referred to here as ageratum enation virus-carrot (AEV-Car). Symptomatic ageratum (Ageratum conyzoides) plants, growing adjacent to the carrot fields, also showed the presence of AEV (AEV-Age). Ageratum yellow leaf curl betasatellite (AYLCB) was also detected in the AEV infected carrot and ageratum samples. AEV-Car and AEV-Age are 95-97% identical in their DNA sequences, represent groups of isolates from the respective plant hosts (carrot and ageratum). Agroinoculation using infectious clones of AEV-Car plus AYLCB or AEV-Age plus AYLCB in carrot, ageratum, tobacco (Nicotiana tabacum) and tomato (Solanum lycopersicum) produced yellow mosaic and curling symptoms in leaves of inoculated plants. Agroinoculation of the two isolates together, along with the betasatellite (AEV-Car plus AEV-Age plus AYLCB) resulted in the enhancement of symptoms in comparison to the plants inoculated with single isolate. Plants with more severe symptoms showed a higher level of viral DNA accumulation, suggesting synergistic interactions between the two isolates of AEV.

Original languageEnglish (US)
Pages (from-to)478-485
Number of pages8
JournalVirus research
Volume178
Issue number2
DOIs
StatePublished - Dec 26 2013

Bibliographical note

Funding Information:
Authors are thankful to Department of Biotechnology, Government of India for financial support. RT is thankful to DST, Govt of India for JC Bose Fellowship. Thanks to Dr. Birendra Singh (Skåne University Hospital, Sweden) for critical comments on the manuscript.

Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.

Keywords

  • Geminivirus variants
  • Silencing suppression
  • Synergism
  • Viral genome
  • Virus host range

Fingerprint Dive into the research topics of 'Molecular characterization and pathogenicity of a carrot (Daucus carota) infecting begomovirus and associated betasatellite from India'. Together they form a unique fingerprint.

Cite this