Abstract
(Chemical Equation Presented) Changing substrates: A mutation that forms a cis-proline-peptide bond in a loop structure close to the active site of an aryl esterase from Pseudomonas fluorescens converts the enzyme into a perhydrolase (see picture). The switch in activity is explained by a new hydrogen bond formed between a backbone carbonyl oxygen atom and the peroxy deacylation intermediate.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2742-2746 |
| Number of pages | 5 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 44 |
| Issue number | 18 |
| DOIs | |
| State | Published - Apr 29 2005 |
Keywords
- Enzyme catalysis
- Hydrolases
- Molecular modeling
- Mutagenesis
- Peroxides
Fingerprint
Dive into the research topics of 'Molecular basis of perhydrolase activity in serine hydrolases'. Together they form a unique fingerprint.Cite this
- APA
- Standard
- Harvard
- Vancouver
- Author
- BIBTEX
- RIS