Modulation of the physicochemical properties of chitosan nanoparticles for optimum delivery of plasmid dna through gill mucosa

Vimal Kumar Sharma, Isha Mutreja, Susmita Mitra

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The gill mucosa is a convenient route for the administration of DNA vaccine to fish and other aquatic organisms. Nanocarriers have been considered for delivery; however, a suitable formulation is required. To devise an appropriate carrier, we have synthesized chitosan nanoparticles entrapping pDNA at N/P ratios of 1:1 and 2.5:1, using ionic gelation and microemulsion (μE) methods. For nanoparticle characterization, the size, morphology, zetapotential, and IR spectra were determined. The efficiency of pDNA entrapment was established by gel retardation assay. On the basis of the investigations, the loaded nanoparticles synthesized by μE method appear to have the required characteristics for effective delivery by this route.

Original languageEnglish (US)
Pages (from-to)191-195
Number of pages5
JournalInternational Journal of Nanoscience
Volume8
Issue number1-2
DOIs
StatePublished - 2009

Keywords

  • Chitosan nanoparticles
  • DNA vaccine
  • Gill mucosa
  • Ion gelation
  • Microemulsion

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