TY - JOUR
T1 - Modulation of the oxidative stress and inflammatory cytokine response by thymoquinone in the collagen induced arthritis in Wistar rats
AU - Umar, Sadiq
AU - Zargan, Jamil
AU - Umar, Khalid
AU - Ahmad, Sayeed
AU - Katiyar, Chandra Kant
AU - Khan, Haider A.
N1 - Funding Information:
Author is grateful to Indian council of medical research, Government of India for providing the financial support in the form of senior research fellowship (SRF).
PY - 2012/4/15
Y1 - 2012/4/15
N2 - Thymoquinone (TQ) is the major active compound derived from Nigella sativa. Our aim of this work was to evaluate the antioxidant and antiarthritic activity of TQ in Wistar rat by collagen induced arthritis (CIA). TQ was administered at a dose of 5 mg kg -1 body weight once daily for 21 days. The effects of treatment in the rats were assessed by biochemical (articular elastase, MPO, LPO, GSH, catalase, SOD and NO), inflammatory mediators (IL-1β, IL-6, TNF-α, IL-10, IFN-γ and PGE 2) and histological studies in joints. TQ was effective in bringing significant changes on all the parameters (articular elastase, MPO, LPO, GSH, catalase, SOD and NO) studied. Oral administration of TQ resulted in significantly reduced the levels of pro-inflammatory mediators (IL-1β, IL-6, TNF-α, IFN-γ and PGE 2) and increased level of IL-10. The protective effects of TQ against RA were also evident from the decrease in arthritis scoring and bone histology. In conclusion, the fact that TQ abolished a number of factors known to be involved in RA pathogenesis indicates that the administration of thymoquinone may have potential value in the treatment of inflammatory disease.
AB - Thymoquinone (TQ) is the major active compound derived from Nigella sativa. Our aim of this work was to evaluate the antioxidant and antiarthritic activity of TQ in Wistar rat by collagen induced arthritis (CIA). TQ was administered at a dose of 5 mg kg -1 body weight once daily for 21 days. The effects of treatment in the rats were assessed by biochemical (articular elastase, MPO, LPO, GSH, catalase, SOD and NO), inflammatory mediators (IL-1β, IL-6, TNF-α, IL-10, IFN-γ and PGE 2) and histological studies in joints. TQ was effective in bringing significant changes on all the parameters (articular elastase, MPO, LPO, GSH, catalase, SOD and NO) studied. Oral administration of TQ resulted in significantly reduced the levels of pro-inflammatory mediators (IL-1β, IL-6, TNF-α, IFN-γ and PGE 2) and increased level of IL-10. The protective effects of TQ against RA were also evident from the decrease in arthritis scoring and bone histology. In conclusion, the fact that TQ abolished a number of factors known to be involved in RA pathogenesis indicates that the administration of thymoquinone may have potential value in the treatment of inflammatory disease.
KW - Collagen induced arthritis
KW - IL-6
KW - Nitric oxide
KW - PGE
KW - TNF-α
KW - Thymoquinone
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U2 - 10.1016/j.cbi.2012.03.003
DO - 10.1016/j.cbi.2012.03.003
M3 - Article
C2 - 22450443
AN - SCOPUS:84859312310
SN - 0009-2797
VL - 197
SP - 40
EP - 46
JO - Chemico-Biological Interactions
JF - Chemico-Biological Interactions
IS - 1
ER -