Modulation of N-methyl-D-aspartate and (R,S)-α-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) responses of spinal nociceptive neurons by a N-terminal fragment of substance P

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The effects of an N-terminal fragment of substance P, substance P-(1-7) [SP-(1-7)], on the responses of dorsal horn nociceptive neurons to N-methyl-D-aspartate (NMDA) and (R,S)-α-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) were tested by combined single-unit extracellular recordings/microiontophoresis. While SP-(1-7) had no effects when applied by itself, it was a potent and long-lasting modulator of both NMDA- and AMPA-mediated excitation of spinal dorsal horn nociceptive neurons. NMDA responses were transiently decreased (by an average of 36% of control at minimum) by SP-(1-7) followed by a more sustained increase (by 76% at maximum). In contrast, AMPA responses were only increased by SP-(1-7) (by 81% at maximum). It is hypothesized that the actions of SP-(1-7) on excitatory amino acid (EAA) responses of dorsal horn nociceptive neurons reflect a novel mechanism by which SP and EAAs interact to modulate pain transmission.

Original languageEnglish (US)
Pages (from-to)441-444
Number of pages4
JournalEuropean Journal of Pharmacology
Volume216
Issue number3
DOIs
StatePublished - Jun 17 1992

Fingerprint

Nociceptors
Propionates
N-Methylaspartate
Substance P
Posterior Horn Cells
Excitatory Amino Acids
substance P (1-7)
bucide
Pain

Keywords

  • (Extracellular recording)
  • AMPA
  • Iontophoresis
  • NMDA (N-methyl-D-aspartate)
  • Nociception
  • Spinal cord
  • Substance P-(1-7)

Cite this

@article{d3c594de20154b139e3e595925273bce,
title = "Modulation of N-methyl-D-aspartate and (R,S)-α-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) responses of spinal nociceptive neurons by a N-terminal fragment of substance P",
abstract = "The effects of an N-terminal fragment of substance P, substance P-(1-7) [SP-(1-7)], on the responses of dorsal horn nociceptive neurons to N-methyl-D-aspartate (NMDA) and (R,S)-α-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) were tested by combined single-unit extracellular recordings/microiontophoresis. While SP-(1-7) had no effects when applied by itself, it was a potent and long-lasting modulator of both NMDA- and AMPA-mediated excitation of spinal dorsal horn nociceptive neurons. NMDA responses were transiently decreased (by an average of 36{\%} of control at minimum) by SP-(1-7) followed by a more sustained increase (by 76{\%} at maximum). In contrast, AMPA responses were only increased by SP-(1-7) (by 81{\%} at maximum). It is hypothesized that the actions of SP-(1-7) on excitatory amino acid (EAA) responses of dorsal horn nociceptive neurons reflect a novel mechanism by which SP and EAAs interact to modulate pain transmission.",
keywords = "(Extracellular recording), AMPA, Iontophoresis, NMDA (N-methyl-D-aspartate), Nociception, Spinal cord, Substance P-(1-7)",
author = "D{\'e}nes Budai and Wilcox, {George L} and Larson, {Alice A}",
year = "1992",
month = "6",
day = "17",
doi = "10.1016/0014-2999(92)90443-8",
language = "English (US)",
volume = "216",
pages = "441--444",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Modulation of N-methyl-D-aspartate and (R,S)-α-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) responses of spinal nociceptive neurons by a N-terminal fragment of substance P

AU - Budai, Dénes

AU - Wilcox, George L

AU - Larson, Alice A

PY - 1992/6/17

Y1 - 1992/6/17

N2 - The effects of an N-terminal fragment of substance P, substance P-(1-7) [SP-(1-7)], on the responses of dorsal horn nociceptive neurons to N-methyl-D-aspartate (NMDA) and (R,S)-α-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) were tested by combined single-unit extracellular recordings/microiontophoresis. While SP-(1-7) had no effects when applied by itself, it was a potent and long-lasting modulator of both NMDA- and AMPA-mediated excitation of spinal dorsal horn nociceptive neurons. NMDA responses were transiently decreased (by an average of 36% of control at minimum) by SP-(1-7) followed by a more sustained increase (by 76% at maximum). In contrast, AMPA responses were only increased by SP-(1-7) (by 81% at maximum). It is hypothesized that the actions of SP-(1-7) on excitatory amino acid (EAA) responses of dorsal horn nociceptive neurons reflect a novel mechanism by which SP and EAAs interact to modulate pain transmission.

AB - The effects of an N-terminal fragment of substance P, substance P-(1-7) [SP-(1-7)], on the responses of dorsal horn nociceptive neurons to N-methyl-D-aspartate (NMDA) and (R,S)-α-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) were tested by combined single-unit extracellular recordings/microiontophoresis. While SP-(1-7) had no effects when applied by itself, it was a potent and long-lasting modulator of both NMDA- and AMPA-mediated excitation of spinal dorsal horn nociceptive neurons. NMDA responses were transiently decreased (by an average of 36% of control at minimum) by SP-(1-7) followed by a more sustained increase (by 76% at maximum). In contrast, AMPA responses were only increased by SP-(1-7) (by 81% at maximum). It is hypothesized that the actions of SP-(1-7) on excitatory amino acid (EAA) responses of dorsal horn nociceptive neurons reflect a novel mechanism by which SP and EAAs interact to modulate pain transmission.

KW - (Extracellular recording)

KW - AMPA

KW - Iontophoresis

KW - NMDA (N-methyl-D-aspartate)

KW - Nociception

KW - Spinal cord

KW - Substance P-(1-7)

UR - http://www.scopus.com/inward/record.url?scp=0026764839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026764839&partnerID=8YFLogxK

U2 - 10.1016/0014-2999(92)90443-8

DO - 10.1016/0014-2999(92)90443-8

M3 - Article

VL - 216

SP - 441

EP - 444

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 3

ER -