Modulation of calprotectin in human keratinocytes by keratinocyte growth factor and interleukin-1α

Mika Bando, Yuka Hiroshima, Masatoshi Kataoka, Mark C. Herzberg, Karen F. Ross, Yasuo Shinohara, Takenori Yamamoto, Toshihiko Nagata, Jun Ichi Kido

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Calprotectin is an antimicrobial complex composed of the S100A8 and S100A9 protein family subunits. Contributing to innate immunity, calprotectin expression is increased by interleukin-1α (IL-1α), which modulates keratinocyte differentiation. Keratinocyte growth factor (KGF) is produced by mesenchymal cells and has a mitogenic activity for epithelial cells. In this study, we investigated the effect of KGF on calprotectin expression in keratinocytes and modulation by IL-1α. Human keratinocytes were cultured with KGF in the presence or absence of a KGF receptor (KGFR) inhibitor or mitogen-activated protein kinase (MAPK) inhibitors. Calprotectin (S100A8/S100A9) expression was determined by northern blotting and enzyme-linked immunosorbent assay, respectively, whereas MAPK phosphorylation was analyzed by western blot analysis. KGF significantly decreased the expression of S100A8/S100A9-specific mRNAs and calprotectin protein. In the presence of KGF, KGFR inhibitor or extracellular-regulated kinase inhibitor restored KGF-downregulated expression of S100A8/S100A9. KGF increased IL-1α expression in keratinocytes, whereas IL-1α increased KGF expression in fibroblasts. Cocultured fibroblast and keratinocytes showed lower S100A8/S100A9 mRNA expression than keratinocytes alone in the presence or absence of IL-1α or KGF. These results suggest that fibroblast-derived KGF reduces or restricts calprotectin expression in keratinocytes, which supports our hypothesis that calprotectin expression in keratinocytes is modulated by factors associated with epithelial-mesenchymal interactions.

Original languageEnglish (US)
Pages (from-to)328-333
Number of pages6
JournalImmunology and Cell Biology
Volume88
Issue number3
DOIs
StatePublished - Mar 1 2010

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Leukocyte L1 Antigen Complex
Fibroblast Growth Factor 7
Interleukin-1
Keratinocytes
Fibroblasts
Mitogen-Activated Protein Kinases
Calgranulin B
Calgranulin A
Messenger RNA
Protein Subunits
Protein Kinase Inhibitors
Innate Immunity
Northern Blotting
Phosphotransferases
Down-Regulation
Western Blotting
Epithelial Cells
Enzyme-Linked Immunosorbent Assay
Phosphorylation

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Modulation of calprotectin in human keratinocytes by keratinocyte growth factor and interleukin-1α. / Bando, Mika; Hiroshima, Yuka; Kataoka, Masatoshi; Herzberg, Mark C.; Ross, Karen F.; Shinohara, Yasuo; Yamamoto, Takenori; Nagata, Toshihiko; Kido, Jun Ichi.

In: Immunology and Cell Biology, Vol. 88, No. 3, 01.03.2010, p. 328-333.

Research output: Contribution to journalArticle

Bando, M, Hiroshima, Y, Kataoka, M, Herzberg, MC, Ross, KF, Shinohara, Y, Yamamoto, T, Nagata, T & Kido, JI 2010, 'Modulation of calprotectin in human keratinocytes by keratinocyte growth factor and interleukin-1α', Immunology and Cell Biology, vol. 88, no. 3, pp. 328-333. https://doi.org/10.1038/icb.2009.104
Bando, Mika ; Hiroshima, Yuka ; Kataoka, Masatoshi ; Herzberg, Mark C. ; Ross, Karen F. ; Shinohara, Yasuo ; Yamamoto, Takenori ; Nagata, Toshihiko ; Kido, Jun Ichi. / Modulation of calprotectin in human keratinocytes by keratinocyte growth factor and interleukin-1α. In: Immunology and Cell Biology. 2010 ; Vol. 88, No. 3. pp. 328-333.
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