Abstract
Opioid receptor activity in neuroblastoma x glioma NG108-15 hybrid cell membranes was attenuated by acid phosphatase purified by high performance liquid chromatography and devoid of protease activity. Treatment of membranes with this phosphatase decreased opioid inhibition of adenylate cyclase and this effect was potentiated by the presence of the opioid agonist during the phosphatase treatment. Phosphatase treatment did not affect the number of opioid receptors but it did alter the distribution of receptors among affinity states, by increasing the percentage of receptors in the low affinity state. The similarities between these effects and desensitization of the opioid receptor, during chronic opioid treatment, are discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1369-1375 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 152 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 16 1988 |
| Externally published | Yes |
Bibliographical note
Funding Information:ACKNOWLEDGMENT: This work was supported in part by grant no. DA-005644 from the National Institute Of Drug Abuse. A.K.L. is the recipient of National Institute of Mental Health Physician Scientist Award no. MH-00582. H.H.L. is the recipient of National Institute of Drug Abuse Career Award no. DA-70554.
