Modification of opioid receptor activity by acid phosphatase in neuroblastoma X glioma NG108-15 hybrid cells

Alan K. Louie, Junni Zhan, Ping Yee Law, Horace H. Loh

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Opioid receptor activity in neuroblastoma x glioma NG108-15 hybrid cell membranes was attenuated by acid phosphatase purified by high performance liquid chromatography and devoid of protease activity. Treatment of membranes with this phosphatase decreased opioid inhibition of adenylate cyclase and this effect was potentiated by the presence of the opioid agonist during the phosphatase treatment. Phosphatase treatment did not affect the number of opioid receptors but it did alter the distribution of receptors among affinity states, by increasing the percentage of receptors in the low affinity state. The similarities between these effects and desensitization of the opioid receptor, during chronic opioid treatment, are discussed.

Original languageEnglish (US)
Pages (from-to)1369-1375
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume152
Issue number3
DOIs
StatePublished - May 16 1988

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