Modeling energy depletion in rat livers using Nash equilibrium metabolic pathway analysis

Angelo Lucia, Emily Ferrarese, Korkut Uygun

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


The current gold standard of Static Cold Storage (SCS), which is static cold storage on ice (about + 4 °C) in a specialized media such as the University of Wisconsin solution (UW), limits storage to few hours for vascular and metabolically active tissues such as the liver and the heart. The liver is arguably the pinnacle of metabolism in human body and therefore metabolic pathway analysis immediately becomes very relevant. In this article, a Nash Equilibrium (NE) approach, which is a first principles approach, is used to model and simulate the static cold storage and warm ischemia of a proposed model of liver cells. Simulations of energy depletion in the liver in static cold storage measured by ATP content and energy charge are presented along with comparisons to experimental data. In addition, conversion of Nash Equilibrium iterations to time are described along with an uncertainty analysis for the parameters in the model. Results in this work show that the Nash Equilibrium approach provides a good match to experimental data for energy depletion and that the uncertainty in model parameters is very small with percent variances less than 0.1%.

Original languageEnglish (US)
Article number3496
JournalScientific reports
Issue number1
StatePublished - Dec 2022

Bibliographical note

Funding Information:
This material is partially based upon work supported by the National Science Foundation under Grant No. EEC 1941543. Support from the US National Institutes of Health (grants R01DK096075 and R01DK114506) and the Shriners Hospitals for Children is gratefully acknowledged.

Publisher Copyright:
© 2022, The Author(s).

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.


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