Model Systems to Study the Chronic, Polymicrobial Infections in Cystic Fibrosis: Current Approaches and Exploring Future Directions

George A. O’Toole; Aurélie Crabbé; Rolf Kümmerli; John J. LiPuma; Jennifer M. Bomberger; Jane C. Davies; Dominique Limoli; Vanessa V. Phelan; James B. Bliska; William H. DePas; Lars E. Dietrich; Thomas H. Hampton; Ryan Hunter; Cezar M. Khursigara; Alexa Price-Whelan; Alix Ashare; Robert A. Cramer; Joanna B. Goldberg; Freya Harrison; Deborah A. Hogan; Michael A. Henson; Dean R. Madden; Jared R. Mayers; Carey Nadell; Dianne Newman; Alice Prince; Damian W. Rivett; Joseph D. Schwartzman; Daniel Schultz; Donald C. Sheppard; Alan R. Smyth; Melanie A. Spero; Bruce A. Stanton; Paul E. Turner; Chris van der Gast; Fiona J. Whelan; Rachel Whitaker; Katrine Whiteson

doi:10.1128/mBio.01763-21

Model Systems to Study the Chronic, Polymicrobial Infections in Cystic Fibrosis: Current Approaches and Exploring Future Directions

George A. O’Toole, Aurélie Crabbé, Rolf Kümmerli, John J. LiPuma, Jennifer M. Bomberger, Jane C. Davies, Dominique Limoli, Vanessa V. Phelan, James B. Bliska, William H. DePas, Lars E. Dietrich, Thomas H. Hampton, Ryan Hunter, Cezar M. Khursigara, Alexa Price-Whelan, Alix Ashare, Robert A. Cramer, Joanna B. Goldberg, Freya Harrison, Deborah A. HoganMichael A. Henson, Dean R. Madden, Jared R. Mayers, Carey Nadell, Dianne Newman, Alice Prince, Damian W. Rivett, Joseph D. Schwartzman, Daniel Schultz, Donald C. Sheppard, Alan R. Smyth, Melanie A. Spero, Bruce A. Stanton, Paul E. Turner, Chris van der Gast, Fiona J. Whelan, Rachel Whitaker, Katrine Whiteson

Microbiology

Research output: Contribution to journal › Review article › peer-review

19 Scopus citations

Abstract

A recent workshop titled “Developing Models to Study Polymicrobial Infections,” sponsored by the Dartmouth Cystic Fibrosis Center (DartCF), explored the development of new models to study the polymicrobial infections associated with the airways of persons with cystic fibrosis (CF). The workshop gathered 351 investigators over two virtual sessions. Here, we present the findings of this workshop, summarize some of the challenges involved with developing such models, and suggest three frameworks to tackle this complex problem. The frameworks proposed here, we believe, could be generally useful in developing new model systems for other infectious diseases. Developing and validating new approaches to study the complex polymicrobial communities in the CF airway could open windows to new therapeutics to treat these recalcitrant infections, as well as uncovering organizing principles applicable to chronic polymicrobial infections more generally.

Original language	English (US)
Article number	e01763-21
Journal	mBio
Volume	12
Issue number	5
DOIs	https://doi.org/10.1128/mBio.01763-21
State	Published - Oct 1 2021

Bibliographical note

Funding Information:
DartCF is supported by the NIH (P30-DK117469) and the Cystic Fibrosis Foundation Research Development Program (STANTO19R0). Additional support was provided by NIH/R01AI155424, NIH/1R01HL152190, CFF/CRAMER19GO, CIHR/PJT-156111, NIH/R35GM128690, NIH/R01DK104847, R01/NIHAI127548, CFF/TURNER19PO, NIH/R01HL136919, CFF/ASHARE21G0, NIH/R35HL18500, NIH/P20GM130454, NIHR Systematic Reviews Program, CFF/WHITEL20A0, NIH/R01AI103369, University of Nottingham Anne McLaren Fellowship, Swiss National Science Foundation/31003A_182499, MRC/MR/ R001898/1, NIH/R01HL151385, NIH/R01AI155424, CFF/BOMBER21R3, CFF/SPERO19F0, NIH/ R01HL136647, Paul G. Allen Frontiers Investigator Award, NIH/R01HL136647, Cystic Fibrosis Trust Strategic Research Centre Award, CF Trust/VIA078, CF Trust/VIAO77, CIHR/FDN/ 159902, and CFF/BOMBER18G0.

Publisher Copyright:
Copyright © 2021 O’Toole et al.

Keywords

Airway
Chronic infection
Cystic fibrosis
Models
Polymicrobial

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/mBio.01763-21

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O’Toole, G. A., Crabbé, A., Kümmerli, R., LiPuma, J. J., Bomberger, J. M., Davies, J. C., Limoli, D., Phelan, V. V., Bliska, J. B., DePas, W. H., Dietrich, L. E., Hampton, T. H., Hunter, R., Khursigara, C. M., Price-Whelan, A., Ashare, A., Cramer, R. A., Goldberg, J. B., Harrison, F., ... Whiteson, K. (2021). Model Systems to Study the Chronic, Polymicrobial Infections in Cystic Fibrosis: Current Approaches and Exploring Future Directions. mBio, 12(5), Article e01763-21. https://doi.org/10.1128/mBio.01763-21

O’Toole, GA, Crabbé, A, Kümmerli, R, LiPuma, JJ, Bomberger, JM, Davies, JC, Limoli, D, Phelan, VV, Bliska, JB, DePas, WH, Dietrich, LE, Hampton, TH, Hunter, R, Khursigara, CM, Price-Whelan, A, Ashare, A, Cramer, RA, Goldberg, JB, Harrison, F, Hogan, DA, Henson, MA, Madden, DR, Mayers, JR, Nadell, C, Newman, D, Prince, A, Rivett, DW, Schwartzman, JD, Schultz, D, Sheppard, DC, Smyth, AR, Spero, MA, Stanton, BA, Turner, PE, van der Gast, C, Whelan, FJ, Whitaker, R & Whiteson, K 2021, 'Model Systems to Study the Chronic, Polymicrobial Infections in Cystic Fibrosis: Current Approaches and Exploring Future Directions', mBio, vol. 12, no. 5, e01763-21. https://doi.org/10.1128/mBio.01763-21

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abstract = "A recent workshop titled “Developing Models to Study Polymicrobial Infections,” sponsored by the Dartmouth Cystic Fibrosis Center (DartCF), explored the development of new models to study the polymicrobial infections associated with the airways of persons with cystic fibrosis (CF). The workshop gathered 351 investigators over two virtual sessions. Here, we present the findings of this workshop, summarize some of the challenges involved with developing such models, and suggest three frameworks to tackle this complex problem. The frameworks proposed here, we believe, could be generally useful in developing new model systems for other infectious diseases. Developing and validating new approaches to study the complex polymicrobial communities in the CF airway could open windows to new therapeutics to treat these recalcitrant infections, as well as uncovering organizing principles applicable to chronic polymicrobial infections more generally.",

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note = "Funding Information: DartCF is supported by the NIH (P30-DK117469) and the Cystic Fibrosis Foundation Research Development Program (STANTO19R0). Additional support was provided by NIH/R01AI155424, NIH/1R01HL152190, CFF/CRAMER19GO, CIHR/PJT-156111, NIH/R35GM128690, NIH/R01DK104847, R01/NIHAI127548, CFF/TURNER19PO, NIH/R01HL136919, CFF/ASHARE21G0, NIH/R35HL18500, NIH/P20GM130454, NIHR Systematic Reviews Program, CFF/WHITEL20A0, NIH/R01AI103369, University of Nottingham Anne McLaren Fellowship, Swiss National Science Foundation/31003A_182499, MRC/MR/ R001898/1, NIH/R01HL151385, NIH/R01AI155424, CFF/BOMBER21R3, CFF/SPERO19F0, NIH/ R01HL136647, Paul G. Allen Frontiers Investigator Award, NIH/R01HL136647, Cystic Fibrosis Trust Strategic Research Centre Award, CF Trust/VIA078, CF Trust/VIAO77, CIHR/FDN/ 159902, and CFF/BOMBER18G0. Publisher Copyright: Copyright {\textcopyright} 2021 O{\textquoteright}Toole et al.",

year = "2021",

month = oct,

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doi = "10.1128/mBio.01763-21",

language = "English (US)",

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AU - Crabbé, Aurélie

AU - Kümmerli, Rolf

AU - LiPuma, John J.

AU - Bomberger, Jennifer M.

AU - Davies, Jane C.

AU - Limoli, Dominique

AU - Phelan, Vanessa V.

AU - Bliska, James B.

AU - DePas, William H.

AU - Dietrich, Lars E.

AU - Hampton, Thomas H.

AU - Hunter, Ryan

AU - Khursigara, Cezar M.

AU - Price-Whelan, Alexa

AU - Ashare, Alix

AU - Cramer, Robert A.

AU - Goldberg, Joanna B.

AU - Harrison, Freya

AU - Hogan, Deborah A.

AU - Henson, Michael A.

AU - Madden, Dean R.

AU - Mayers, Jared R.

AU - Nadell, Carey

AU - Newman, Dianne

AU - Prince, Alice

AU - Rivett, Damian W.

AU - Schwartzman, Joseph D.

AU - Schultz, Daniel

AU - Sheppard, Donald C.

AU - Smyth, Alan R.

AU - Spero, Melanie A.

AU - Stanton, Bruce A.

AU - Turner, Paul E.

AU - van der Gast, Chris

AU - Whelan, Fiona J.

AU - Whitaker, Rachel

AU - Whiteson, Katrine

N1 - Funding Information: DartCF is supported by the NIH (P30-DK117469) and the Cystic Fibrosis Foundation Research Development Program (STANTO19R0). Additional support was provided by NIH/R01AI155424, NIH/1R01HL152190, CFF/CRAMER19GO, CIHR/PJT-156111, NIH/R35GM128690, NIH/R01DK104847, R01/NIHAI127548, CFF/TURNER19PO, NIH/R01HL136919, CFF/ASHARE21G0, NIH/R35HL18500, NIH/P20GM130454, NIHR Systematic Reviews Program, CFF/WHITEL20A0, NIH/R01AI103369, University of Nottingham Anne McLaren Fellowship, Swiss National Science Foundation/31003A_182499, MRC/MR/ R001898/1, NIH/R01HL151385, NIH/R01AI155424, CFF/BOMBER21R3, CFF/SPERO19F0, NIH/ R01HL136647, Paul G. Allen Frontiers Investigator Award, NIH/R01HL136647, Cystic Fibrosis Trust Strategic Research Centre Award, CF Trust/VIA078, CF Trust/VIAO77, CIHR/FDN/ 159902, and CFF/BOMBER18G0. Publisher Copyright: Copyright © 2021 O’Toole et al.

PY - 2021/10/1

Y1 - 2021/10/1

N2 - A recent workshop titled “Developing Models to Study Polymicrobial Infections,” sponsored by the Dartmouth Cystic Fibrosis Center (DartCF), explored the development of new models to study the polymicrobial infections associated with the airways of persons with cystic fibrosis (CF). The workshop gathered 351 investigators over two virtual sessions. Here, we present the findings of this workshop, summarize some of the challenges involved with developing such models, and suggest three frameworks to tackle this complex problem. The frameworks proposed here, we believe, could be generally useful in developing new model systems for other infectious diseases. Developing and validating new approaches to study the complex polymicrobial communities in the CF airway could open windows to new therapeutics to treat these recalcitrant infections, as well as uncovering organizing principles applicable to chronic polymicrobial infections more generally.

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KW - Airway

KW - Chronic infection

KW - Cystic fibrosis

KW - Models

KW - Polymicrobial

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JO - mBio

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Model Systems to Study the Chronic, Polymicrobial Infections in Cystic Fibrosis: Current Approaches and Exploring Future Directions

Abstract

Bibliographical note

Keywords

UN SDGs

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