Mobilization of seed storage lipid by Arabidopsis seedlings is retarded in the presence of exogenous sugars

Jennifer P.C. To, Wolf Dieter Reiter, Susan I. Gibson

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background: Soluble sugar levels must be closely regulated in germinating seeds to ensure an adequate supply of energy and building materials for the developing seedling. Studies on germinating cereal seeds indicate that production of sugars from starch is inhibited by increasing sugar levels. Although numerous studies have focused on the regulation of starch metabolism, very few studies have addressed the control of storage lipid metabolism by germinating oilseeds. Results: Mobilization of storage lipid by germinating seeds of the model oilseed plant Arabidopsis thaliana (L.) Heynh. occurs at a greatly reduced rate in the presence of exogenous glucose or mannose, but not in the presence of equi-molar 3-O-methylglucose or sorbitol. The sugar-insensitive5-1/abscisic acid-insensitive4-101 (sis5-1/abi4-101) mutant is resistant to glucose inhibition of seed storage lipid mobilization. Wild-type seedlings become insensitive to glucose inhibition of storage lipid breakdown within 3 days of the start of imbibition. Conclusions: Growth in the presence of exogenous glucose significantly retards mobilization of seed storage lipid in germinating seeds from wild-type Arabidopsis. This effect is not solely due to the osmotic potential of the media, as substantially higher concentrations of sorbitol than of glucose are required to exert significant effects on lipid breakdown. The inhibitory effect of glucose on lipid breakdown is limited to a narrow developmental window, suggesting that completion of some critical metabolic transition results in loss of sensitivity to the inhibitory effect of glucose on lipid breakdown.

Original languageEnglish (US)
Article number4
JournalBMC plant biology
Volume2
DOIs
StatePublished - May 7 2002

Fingerprint

seed storage
Arabidopsis
sugars
glucose
seedlings
lipids
sorbitol
seeds
seed productivity
starch
Equus
oilseed crops
oilseeds
imbibition
mannose
osmotic pressure
lipid metabolism
abscisic acid
Arabidopsis thaliana
mutants

Cite this

Mobilization of seed storage lipid by Arabidopsis seedlings is retarded in the presence of exogenous sugars. / To, Jennifer P.C.; Reiter, Wolf Dieter; Gibson, Susan I.

In: BMC plant biology, Vol. 2, 4, 07.05.2002.

Research output: Contribution to journalArticle

@article{da08765f45d049b2a9a02e088a1c9278,
title = "Mobilization of seed storage lipid by Arabidopsis seedlings is retarded in the presence of exogenous sugars",
abstract = "Background: Soluble sugar levels must be closely regulated in germinating seeds to ensure an adequate supply of energy and building materials for the developing seedling. Studies on germinating cereal seeds indicate that production of sugars from starch is inhibited by increasing sugar levels. Although numerous studies have focused on the regulation of starch metabolism, very few studies have addressed the control of storage lipid metabolism by germinating oilseeds. Results: Mobilization of storage lipid by germinating seeds of the model oilseed plant Arabidopsis thaliana (L.) Heynh. occurs at a greatly reduced rate in the presence of exogenous glucose or mannose, but not in the presence of equi-molar 3-O-methylglucose or sorbitol. The sugar-insensitive5-1/abscisic acid-insensitive4-101 (sis5-1/abi4-101) mutant is resistant to glucose inhibition of seed storage lipid mobilization. Wild-type seedlings become insensitive to glucose inhibition of storage lipid breakdown within 3 days of the start of imbibition. Conclusions: Growth in the presence of exogenous glucose significantly retards mobilization of seed storage lipid in germinating seeds from wild-type Arabidopsis. This effect is not solely due to the osmotic potential of the media, as substantially higher concentrations of sorbitol than of glucose are required to exert significant effects on lipid breakdown. The inhibitory effect of glucose on lipid breakdown is limited to a narrow developmental window, suggesting that completion of some critical metabolic transition results in loss of sensitivity to the inhibitory effect of glucose on lipid breakdown.",
author = "To, {Jennifer P.C.} and Reiter, {Wolf Dieter} and Gibson, {Susan I.}",
year = "2002",
month = "5",
day = "7",
doi = "10.1186/1471-2229-2-4",
language = "English (US)",
volume = "2",
journal = "BMC Plant Biology",
issn = "1471-2229",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Mobilization of seed storage lipid by Arabidopsis seedlings is retarded in the presence of exogenous sugars

AU - To, Jennifer P.C.

AU - Reiter, Wolf Dieter

AU - Gibson, Susan I.

PY - 2002/5/7

Y1 - 2002/5/7

N2 - Background: Soluble sugar levels must be closely regulated in germinating seeds to ensure an adequate supply of energy and building materials for the developing seedling. Studies on germinating cereal seeds indicate that production of sugars from starch is inhibited by increasing sugar levels. Although numerous studies have focused on the regulation of starch metabolism, very few studies have addressed the control of storage lipid metabolism by germinating oilseeds. Results: Mobilization of storage lipid by germinating seeds of the model oilseed plant Arabidopsis thaliana (L.) Heynh. occurs at a greatly reduced rate in the presence of exogenous glucose or mannose, but not in the presence of equi-molar 3-O-methylglucose or sorbitol. The sugar-insensitive5-1/abscisic acid-insensitive4-101 (sis5-1/abi4-101) mutant is resistant to glucose inhibition of seed storage lipid mobilization. Wild-type seedlings become insensitive to glucose inhibition of storage lipid breakdown within 3 days of the start of imbibition. Conclusions: Growth in the presence of exogenous glucose significantly retards mobilization of seed storage lipid in germinating seeds from wild-type Arabidopsis. This effect is not solely due to the osmotic potential of the media, as substantially higher concentrations of sorbitol than of glucose are required to exert significant effects on lipid breakdown. The inhibitory effect of glucose on lipid breakdown is limited to a narrow developmental window, suggesting that completion of some critical metabolic transition results in loss of sensitivity to the inhibitory effect of glucose on lipid breakdown.

AB - Background: Soluble sugar levels must be closely regulated in germinating seeds to ensure an adequate supply of energy and building materials for the developing seedling. Studies on germinating cereal seeds indicate that production of sugars from starch is inhibited by increasing sugar levels. Although numerous studies have focused on the regulation of starch metabolism, very few studies have addressed the control of storage lipid metabolism by germinating oilseeds. Results: Mobilization of storage lipid by germinating seeds of the model oilseed plant Arabidopsis thaliana (L.) Heynh. occurs at a greatly reduced rate in the presence of exogenous glucose or mannose, but not in the presence of equi-molar 3-O-methylglucose or sorbitol. The sugar-insensitive5-1/abscisic acid-insensitive4-101 (sis5-1/abi4-101) mutant is resistant to glucose inhibition of seed storage lipid mobilization. Wild-type seedlings become insensitive to glucose inhibition of storage lipid breakdown within 3 days of the start of imbibition. Conclusions: Growth in the presence of exogenous glucose significantly retards mobilization of seed storage lipid in germinating seeds from wild-type Arabidopsis. This effect is not solely due to the osmotic potential of the media, as substantially higher concentrations of sorbitol than of glucose are required to exert significant effects on lipid breakdown. The inhibitory effect of glucose on lipid breakdown is limited to a narrow developmental window, suggesting that completion of some critical metabolic transition results in loss of sensitivity to the inhibitory effect of glucose on lipid breakdown.

UR - http://www.scopus.com/inward/record.url?scp=0038707921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038707921&partnerID=8YFLogxK

U2 - 10.1186/1471-2229-2-4

DO - 10.1186/1471-2229-2-4

M3 - Article

C2 - 11996676

AN - SCOPUS:0038707921

VL - 2

JO - BMC Plant Biology

JF - BMC Plant Biology

SN - 1471-2229

M1 - 4

ER -