Mixture toxicity of three toxicants with similar and dissimilar modes of action to Daphnia magna

Kristian Syberg, Anders Elleby, Henrik Pedersen, Nina Cedergreen, Valery E. Forbes

Research output: Contribution to journalArticlepeer-review

89 Scopus citations


Mixture toxicity of similar- and dissimilar-acting toxicants can be predicted by the models concentration addition (CA) and independent action (IA) using single substance toxicity data. Knowledge of the toxicants mode of action is thus required in order to use the models. In order to test the predictive capability of the models, we conducted Daphnia magna 48 h immobilization experiments with three toxicants with known modes of action (dimethoate, pirimicarb and linear alkyl benzene sulfonate) singly, and in binary and ternary mixtures. Our results indicate that CA and IA predict binary mixtures of similar- and dissimilar-acting toxicants equally well. CA and IA also equally predicted the ternary mixture consisting of both similar- and dissimilar-acting chemicals. The paper discusses the concept of mode of action and the implications the definition of mode of action has on the choice of reference model for mixture toxicity studies.

Original languageEnglish (US)
Pages (from-to)428-436
Number of pages9
JournalEcotoxicology and Environmental Safety
Issue number3
StatePublished - Mar 2008

Bibliographical note

Funding Information:
Funding source: Basic funding from Roskilde University supported this study.


  • Concentration addition
  • Daphnia magna
  • Dimethoate
  • Independent action
  • Isobole method
  • Linear alkyl benzene sulfonate
  • Mixture toxicity
  • Mode of action
  • Pirimicarb


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