TY - JOUR
T1 - Mixotrophic culture of Chaetoceros sp. and the synergistic carbon and energy metabolism
AU - Shan, Shengzhou
AU - Wang, Shanshan
AU - Yan, Xi
AU - Chen, Kang
AU - Liang, Li
AU - Li, Xiaohui
AU - Zhou, Chengxu
AU - Yan, Xiaojun
AU - Ruan, Roger
AU - Cheng, Pengfei
N1 - Publisher Copyright:
© 2023 Elsevier Ltd
PY - 2023/12
Y1 - 2023/12
N2 - This research studied the metabolic mechanism of the mixotrophic Chaetoceros sp. The results showed this alga had the highest cell density and growth rate of 47.72 × 105 cells mL−1 and 0.41 d-1, respectively, with a maximum dry weight of 2.90 g/L, when compared to photoautotrophic and photoheterotrophic modes. Compared to photoheterotrophy, transcriptomics results showed the Rubisco, PGK, and GAPDH related genes were separately up-regulated by 1.03, 2.36, and 1.36 times in CBB cycle in mixotrophic mode, suggesting intermediate metabolites of EMP and PPP can enter the chloroplast via transporter proteins, or membrane permeation, and feedback inhibition regulates the reduction of multiple reactions in CBB cycle. Chaetoceros sp. achieves high biomass by utilizing ATP and carbon structures from EMP and PPP pathways, and the addition of NaHCO3 leads to an up-regulation of CBB cycle for the mixotrophic alga, resulting in higher biomass compared to the photoheterotrophic mode.
AB - This research studied the metabolic mechanism of the mixotrophic Chaetoceros sp. The results showed this alga had the highest cell density and growth rate of 47.72 × 105 cells mL−1 and 0.41 d-1, respectively, with a maximum dry weight of 2.90 g/L, when compared to photoautotrophic and photoheterotrophic modes. Compared to photoheterotrophy, transcriptomics results showed the Rubisco, PGK, and GAPDH related genes were separately up-regulated by 1.03, 2.36, and 1.36 times in CBB cycle in mixotrophic mode, suggesting intermediate metabolites of EMP and PPP can enter the chloroplast via transporter proteins, or membrane permeation, and feedback inhibition regulates the reduction of multiple reactions in CBB cycle. Chaetoceros sp. achieves high biomass by utilizing ATP and carbon structures from EMP and PPP pathways, and the addition of NaHCO3 leads to an up-regulation of CBB cycle for the mixotrophic alga, resulting in higher biomass compared to the photoheterotrophic mode.
KW - Carbon metabolism
KW - Chaetoceros sp.
KW - Energy metabolism
KW - Mixotrophic culture
KW - Synergistic mechanism
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U2 - 10.1016/j.biortech.2023.129912
DO - 10.1016/j.biortech.2023.129912
M3 - Article
C2 - 37879446
AN - SCOPUS:85175053346
SN - 0960-8524
VL - 390
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 129912
ER -