Mixotrophic culture of bait microalgae for biomass and nutrients accumulation and their synergistic carbon metabolism

Yahui Bo; Ruirui Chu; Danni Sun; Xiangyuan Deng; Chengxu Zhou; Xiaojun Yan; Roger Ruan; Pengfei Cheng

doi:10.1016/j.biortech.2022.128301

Mixotrophic culture of bait microalgae for biomass and nutrients accumulation and their synergistic carbon metabolism

Yahui Bo, Ruirui Chu, Danni Sun, Xiangyuan Deng, Chengxu Zhou, Xiaojun Yan, Roger Ruan, Pengfei Cheng

Bioproducts and Biosystems Engineering

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Microalgae cannot meet the bait demand for aquaculture due to light intensity limitation and other disadvantageous conditions. This research selected 6 mixotrophic microalgae, and the optimal strains and organic carbon were screened. The results showed that Thalassiosira pseudonana and Chlorella sp. are suitable for mixotrophic culture. The maximum cell density of Thalassiosira pseudonana was found to be 1.67 times than that of the photoautotrophic group when glycerol was added. The maximum cell density of Chlorella sp. with acetic acid was 1.69 times than that of the photoautotrophic group. When the concentration of acetic acid was 5.0 g·L^-1 and the concentration of KNO₃ was 0.2 g·L^-1, the maximum biomass of Chlorella sp. could reach 3.54 × 10⁷ cells·mL⁻¹; the maximum biomass of Thalassiosira pseudonana was 5.53 × 10⁶ cells·mL⁻¹ with 10.0 g·L^-1 glycerol and 0.2 g·L^-1 KNO₃. Metabolomic analysis further revealed that mixotrophic bait microalgae could promote the accumulation of lipids and amino acids.

Original language	English (US)
Article number	128301
Journal	Bioresource Technology
Volume	367
DOIs	https://doi.org/10.1016/j.biortech.2022.128301
State	Published - Jan 2023

Bibliographical note

Funding Information:
This research was supported in part by grants from the National Natural Science Foundation of China (32170369), the Natural Science Foundation of Zhejiang Province (LZJWY22B070001), the Fundamental Research Funds for the Provincial Universities (SJLY2020007), the LiDakSum Marine Biopharmaceutical Development Fund, and the National 111 Project of China, University of Minnesota MnDrive Environment Program MNE12, and University of Minnesota Center for Biorefining.

Publisher Copyright:
© 2022 Elsevier Ltd

Keywords

Bait microalgae
Culture conditions
Metabolomic analysis
Mixotrophic culture
Organic carbon

PubMed: MeSH publication types

Journal Article

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10.1016/j.biortech.2022.128301

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title = "Mixotrophic culture of bait microalgae for biomass and nutrients accumulation and their synergistic carbon metabolism",

abstract = "Microalgae cannot meet the bait demand for aquaculture due to light intensity limitation and other disadvantageous conditions. This research selected 6 mixotrophic microalgae, and the optimal strains and organic carbon were screened. The results showed that Thalassiosira pseudonana and Chlorella sp. are suitable for mixotrophic culture. The maximum cell density of Thalassiosira pseudonana was found to be 1.67 times than that of the photoautotrophic group when glycerol was added. The maximum cell density of Chlorella sp. with acetic acid was 1.69 times than that of the photoautotrophic group. When the concentration of acetic acid was 5.0 g·L-1 and the concentration of KNO3 was 0.2 g·L-1, the maximum biomass of Chlorella sp. could reach 3.54 × 107 cells·mL−1; the maximum biomass of Thalassiosira pseudonana was 5.53 × 106 cells·mL−1 with 10.0 g·L-1 glycerol and 0.2 g·L-1 KNO3. Metabolomic analysis further revealed that mixotrophic bait microalgae could promote the accumulation of lipids and amino acids.",

keywords = "Bait microalgae, Culture conditions, Metabolomic analysis, Mixotrophic culture, Organic carbon",

author = "Yahui Bo and Ruirui Chu and Danni Sun and Xiangyuan Deng and Chengxu Zhou and Xiaojun Yan and Roger Ruan and Pengfei Cheng",

note = "Funding Information: This research was supported in part by grants from the National Natural Science Foundation of China (32170369), the Natural Science Foundation of Zhejiang Province (LZJWY22B070001), the Fundamental Research Funds for the Provincial Universities (SJLY2020007), the LiDakSum Marine Biopharmaceutical Development Fund, and the National 111 Project of China, University of Minnesota MnDrive Environment Program MNE12, and University of Minnesota Center for Biorefining. Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd",

year = "2023",

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doi = "10.1016/j.biortech.2022.128301",

language = "English (US)",

volume = "367",

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T1 - Mixotrophic culture of bait microalgae for biomass and nutrients accumulation and their synergistic carbon metabolism

AU - Bo, Yahui

AU - Chu, Ruirui

AU - Sun, Danni

AU - Deng, Xiangyuan

AU - Zhou, Chengxu

AU - Yan, Xiaojun

AU - Ruan, Roger

AU - Cheng, Pengfei

N1 - Funding Information: This research was supported in part by grants from the National Natural Science Foundation of China (32170369), the Natural Science Foundation of Zhejiang Province (LZJWY22B070001), the Fundamental Research Funds for the Provincial Universities (SJLY2020007), the LiDakSum Marine Biopharmaceutical Development Fund, and the National 111 Project of China, University of Minnesota MnDrive Environment Program MNE12, and University of Minnesota Center for Biorefining. Publisher Copyright: © 2022 Elsevier Ltd

PY - 2023/1

Y1 - 2023/1

N2 - Microalgae cannot meet the bait demand for aquaculture due to light intensity limitation and other disadvantageous conditions. This research selected 6 mixotrophic microalgae, and the optimal strains and organic carbon were screened. The results showed that Thalassiosira pseudonana and Chlorella sp. are suitable for mixotrophic culture. The maximum cell density of Thalassiosira pseudonana was found to be 1.67 times than that of the photoautotrophic group when glycerol was added. The maximum cell density of Chlorella sp. with acetic acid was 1.69 times than that of the photoautotrophic group. When the concentration of acetic acid was 5.0 g·L-1 and the concentration of KNO3 was 0.2 g·L-1, the maximum biomass of Chlorella sp. could reach 3.54 × 107 cells·mL−1; the maximum biomass of Thalassiosira pseudonana was 5.53 × 106 cells·mL−1 with 10.0 g·L-1 glycerol and 0.2 g·L-1 KNO3. Metabolomic analysis further revealed that mixotrophic bait microalgae could promote the accumulation of lipids and amino acids.

AB - Microalgae cannot meet the bait demand for aquaculture due to light intensity limitation and other disadvantageous conditions. This research selected 6 mixotrophic microalgae, and the optimal strains and organic carbon were screened. The results showed that Thalassiosira pseudonana and Chlorella sp. are suitable for mixotrophic culture. The maximum cell density of Thalassiosira pseudonana was found to be 1.67 times than that of the photoautotrophic group when glycerol was added. The maximum cell density of Chlorella sp. with acetic acid was 1.69 times than that of the photoautotrophic group. When the concentration of acetic acid was 5.0 g·L-1 and the concentration of KNO3 was 0.2 g·L-1, the maximum biomass of Chlorella sp. could reach 3.54 × 107 cells·mL−1; the maximum biomass of Thalassiosira pseudonana was 5.53 × 106 cells·mL−1 with 10.0 g·L-1 glycerol and 0.2 g·L-1 KNO3. Metabolomic analysis further revealed that mixotrophic bait microalgae could promote the accumulation of lipids and amino acids.

KW - Bait microalgae

KW - Culture conditions

KW - Metabolomic analysis

KW - Mixotrophic culture

KW - Organic carbon

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Mixotrophic culture of bait microalgae for biomass and nutrients accumulation and their synergistic carbon metabolism

Abstract

Bibliographical note

Keywords

PubMed: MeSH publication types

UN SDGs

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