Mixotrophic culture of bait microalgae for biomass and nutrients accumulation and their synergistic carbon metabolism

Yahui Bo, Ruirui Chu, Danni Sun, Xiangyuan Deng, Chengxu Zhou, Xiaojun Yan, Roger Ruan, Pengfei Cheng

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Microalgae cannot meet the bait demand for aquaculture due to light intensity limitation and other disadvantageous conditions. This research selected 6 mixotrophic microalgae, and the optimal strains and organic carbon were screened. The results showed that Thalassiosira pseudonana and Chlorella sp. are suitable for mixotrophic culture. The maximum cell density of Thalassiosira pseudonana was found to be 1.67 times than that of the photoautotrophic group when glycerol was added. The maximum cell density of Chlorella sp. with acetic acid was 1.69 times than that of the photoautotrophic group. When the concentration of acetic acid was 5.0 g·L-1 and the concentration of KNO3 was 0.2 g·L-1, the maximum biomass of Chlorella sp. could reach 3.54 × 107 cells·mL−1; the maximum biomass of Thalassiosira pseudonana was 5.53 × 106 cells·mL−1 with 10.0 g·L-1 glycerol and 0.2 g·L-1 KNO3. Metabolomic analysis further revealed that mixotrophic bait microalgae could promote the accumulation of lipids and amino acids.

Original languageEnglish (US)
Article number128301
JournalBioresource Technology
StatePublished - Jan 2023

Bibliographical note

Funding Information:
This research was supported in part by grants from the National Natural Science Foundation of China (32170369), the Natural Science Foundation of Zhejiang Province (LZJWY22B070001), the Fundamental Research Funds for the Provincial Universities (SJLY2020007), the LiDakSum Marine Biopharmaceutical Development Fund, and the National 111 Project of China, University of Minnesota MnDrive Environment Program MNE12, and University of Minnesota Center for Biorefining.

Publisher Copyright:
© 2022 Elsevier Ltd


  • Bait microalgae
  • Culture conditions
  • Metabolomic analysis
  • Mixotrophic culture
  • Organic carbon

PubMed: MeSH publication types

  • Journal Article


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