Mixing effects on light exposure in a large-lake epilimnion: A preliminary dual-dye study

Elizabeth C Austin-Minor, Jay A Austin, Luni Sun, Lance Gauer, Richard C. Zimmerman, Kenneth Mopper

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Light plays a critically important role in driving photo- and biogeochemistry of aquatic systems, and our ability to measure and model submarine light fields is fairly sophisticated. However, previous approaches have relied almost entirely upon Eulerian rather than Lagrangian measurements. Such approaches can provide good estimates of the instantaneous light field but do not necessarily reflect the doses of light experienced by particles and chemical species passively transported by physical processes within a lake or ocean surface-layer. Here, a novel dual-dye approach, used as a Lagrangian light dosimeter, was applied in Lake Superior’s surface mixed layer to determine the importance of mixing in affecting the light dose experienced by particles passively mixed within is this layer. Two fluorescent dyes were deployed in the water column in a known ratio; one of the dyes (fluorescein) was sensitive to light exposure and one (rhodamine WT) was relatively photostable. The photochemistry of the dyes (i.e., the quantum yield of fluorescence bleaching at different wavelengths) and the role of matrix effects on dye response in a natural water system were explored with laboratory experiments. Decay equations for fluorescein and rhodamine WT fluorescence were determined from controlled irradiations of dye solutions with natural sunlight. The mixed-layer light dose (from 460 nm to 510 nm) was then traced for two dye deployments in Lake Superior. The results show the importance of vertical mixing on the light dose within Lake Superior’s surface layer on time scales of hours.

Original languageEnglish (US)
Pages (from-to)542-554
Number of pages13
JournalLimnology and Oceanography: Methods
Issue number8
StatePublished - Aug 1 2016

Bibliographical note

Funding Information:
We thank the captain and crew of the R/V Blue Heron and Lucas Gloege and Dan Titze for assistance in deploying and tracing the dyes and measuring SPAR. Thanks also to Hongyu Li for help with fluorometry and the matrix effect experiment. Thanks to Sam Kelly for helpful comments concerning effective depths in an early version of this manuscript. This work was funded by NSF (OCE-1235379 to E.C.M. and J.A. and OCE-1235005 to K.M.).

Publisher Copyright:
© 2016 Association for the Sciences of Limnology and Oceanography.


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